Overview

This COLOR book Includes Introduction and Five Multi-Week Wet Lab Projects Full List of Required Materials and Catalog Numbers, Detailed Setup Instructions for Each Project, Quarter and Semester Schedules (8 and 12 week)1. Introduction, Safety, Pipettors and Practical 2. Create a Molecular Size Standard using PCR 3. Prokaryote Sequencing 4. Describe a Human Gene Sequence and Design PCR Primers 5. Primer Rehydration and Human DNA Extraction 6. Restriction Digest of PCR products. These projects use substantial evidence-based grading, meaning that answers vary based on selections made within each project. The black and white student edition is far less expensive, so if you are student, that is your best bet.....Because these labs are graded using unique evidence generated by the student, the potential for cheating is substantially reduced. General equipment needed to run these labs: Thermal cycler (PCR machine); preferably with a 96 well head, Pipettes: Typically a set of three (2-20, 20-200, 200-1,000 l) per two to three students, Small microfuges (usually 600 rpm speed) with 1.5 - 2.0 ml tube and 0.2 ml PCR strip rotors, Tabletop centrifuge (usually capable of 14,000 rpm) with a 1.5/2.0 ml tube rotor. Electrophoresis power supply and gel rig with matching trays and combs. UV documentation system10X dissecting scopes for prokaryote phenotype identification (project 3; 1 per 6 students recommended)Tabletop heat block or water bath, General lab supplies such as beakers, microwave, glassware, etc. Approximate cost $25 per student can be reduced if needed, but is not recommended.

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