Overview

In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Full Product Details

Author:   Joseph LeDoux
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   3rd ed. 2008
Volume:   433
Dimensions:   Width: 15.50cm , Height: 2.10cm , Length: 23.50cm
Weight:   0.611kg
ISBN:  

9781627039567

ISBN 10:   1627039562
Pages:   386
Publication Date:   02 November 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preparation and Quantification of Pseudotyped Retroviral Vector.- Production of Retroviral Vectors for Clinical Use.- Methods for the Production of Helper-Dependent Adenoviral Vectors.- Methods for the Production of First Generation Adenoviral Vectors.- Large-Scale Production of Recombinant Adeno-Associated Viral Vectors.- Construction and Production of Recombinant Herpes Simplex Virus Vectors.- Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation.- Chitosan Nanoparticle-Mediated Gene Transfer.- PEG–PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues.- Non-Viral Gene Delivery with Cationic Liposome–DNA Complexes.- Applications of Lentiviral Vectors in Noninvasive Molecular Imaging.- Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia.- Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells.- Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo.- Oncolytic Adenoviruses for Cancer Gene Therapy.- Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy.- Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells.- Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells.- Nanoparticle-Mediated Gene Delivery to the Lung.- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype.- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription.- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.

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