Overview

Full Product Details

Author:   Jeffrey R. Morgan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd Revised edition
Volume:   v. 69
Dimensions:   Width: 15.20cm , Height: 3.60cm , Length: 22.90cm
Weight:   0.852kg
ISBN:  

9780896038691

ISBN 10:   0896038696
Pages:   536
Publication Date:   10 October 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Contents Agarose and Polyacrylamide Gel Electrophoresis Andrea M. Guilliatt Internal Labeling of DNA Probes Ralph Rapley and Bimal D. M. Theophilus End-Labeling of DNA Probes Adrian J. Harwood Southern Blotting of Agarose Gels by Capillary Transfer Ralph Rapley and Ian J. Williams Restriction Fragment Length Polymorphism Mohammad S. Enayat PCR: Principles, Procedures, and Parameters Nicola Louise Jones Allele-Specific Oligonucleotide PCR Elaine K. Green Long-Range PCR, Peter A. Davies and George Gray Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing Anu Wartiovaara and Ann-Christine Syvanen Cycle Sequencing of PCR Products G. K. Surdhar Fluorescent In Situ Hybridization Sara A. Dyer and Elaine K. Green. The Protein Truncation Test Carol A. Hardy Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli Denaturing Gradient Gel Electrophoresis Yvonne Wallis Conformation-Sensitive Gel Electrophoresis Ian J. Williams and Anne C. Goodeve SSCP/Heteroduplex Analysis Andrew J. Wallace Cleavase(R) Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection Laura Heisler and Chao-Hung Lee Automated Genotyping Using the DNA MassArray(tm) Technology Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Koster An Introduction to Bioinformatics Henry Brzeski

Reviews

Each protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies. - E-Streams From Reviews of the First Edition: extremely impressive a must, even for researchers who are already familiar with gene transfer techniques - Molecular Medicine Today succeeds admirably - Genetical Research

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