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Author:   B. D. Hames ,  Stephen J. Higgins
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No.161 & 162
Dimensions:   Width: 15.00cm , Height: 4.70cm , Length: 23.00cm
Weight:   1.490kg
ISBN:  

9780199636136

ISBN 10:   0199636133
Pages:   732
Publication Date:   01 December 1995
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Volume 1: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of synthetic oligodeoxynucleotide probes; Probe preparation of PCR; Synthesis of labelled riboprobes; Antibody probes. Volume 2: Hybridization strategy; Screening recombinant DNA libraries by hybridization and amplification; Screening human recombinant libraries with nucleic acid probes; Antibody screening of bacterial expression systems; Isolation of cDNA clones by direct expression in mammalian cells; Genomic mapping and gene structure analysis; Analysis of gene expression by blotting techniques; In situ hybridization of nucleic acid probes to cellular RNA; Applications of chromosomal in situ hybridization; Analysis of gene expression analysis by PCR: a quantitative approach; Analysis of protein expression by immunocytochemistry; Gene targeting in mammalian cells; Directed mutagenesis and mutant analysis; Human gene mutational analysis.

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