Overview

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Full Product Details

Author:   William C. Heiser
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   245
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   1.380kg
ISBN:  

9781588290861

ISBN 10:   1588290867
Pages:   301
Publication Date:   28 October 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Delivery Using Chemical Methods.- Chemical Methods for DNA Delivery.- Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran.- DNA Delivery to Cells in Culture Using Peptides.- DNA Delivery to Cells in Culture Using PNA Clamps.- Dendrimer-Mediated Cell Transfection in Vitro.- DNA Delivery to Cells in Culture Using Cationic Liposomes.- Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium.- Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium.- Delivery of DNA to Tumor Cells Using Cationic Liposomes.- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes.- Delivery Using Physical Methods.- Gene Delivery Using Physical Methods.- Gene Delivery to Mammalian Cells by Microinjection.- Delivery of DNA to Cells in Culture Using Particle Bombardment.- Delivery of DNA to Skin by Particle Bombardment.- Biolistic Transfection of Cultured Organotypic Brain Slices.- Efficient Electroporation of Mammalian Cells in Culture.- Delivery of DNA to Skin by Electroporation.- In Vivo DNA Electransfer in Skeletal Muscle.- Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo.- Hydrodynamic Delivery of DNA.- Naked DNA Gene Transfer in Mammalian Cells.- Microparticle Delivery of Plasmid DNA to Mammalian Cells.- DNA Delivery to Cells in Culture Using Ultrasound.- DNA Delivery to Cells In Vivo by Ultrasound.

Reviews

For Volumes 1 & 2 This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike. - Medical Oncology Volume 1 ...provides insights into the choices available in this important area of biology and medicine. - Microbiology Today

For Volumes 1 & 2 This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike. - Medical Oncology Volume 1 ...provides insights into the choices available in this important area of biology and medicine. - Microbiology Today

For Volumes 1 & 2 This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike. Medical Oncology Volume 1 provides insights into the choices available in this important area of biology and medicine. Microbiology Today

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