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Author:   T. A. Brown (University of Manchester, UK)
Publisher:   John Wiley & Sons Inc
Imprint:   John Wiley & Sons Inc
Edition:   9th edition
Dimensions:   Width: 16.50cm , Height: 2.50cm , Length: 23.90cm
Weight:   0.816kg
ISBN:  

9781394292561

ISBN 10:   1394292562
Pages:   448
Publication Date:   06 November 2025
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

Preface to the Ninth Edition xv About the Companion Website xvii Part I The Basic Principles of Gene Cloning and DNA Analysis 1 1 Why Gene Cloning and DNA Analysis Are Important? 3 2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15 3 Purification of DNA from Living Cells 29 4 Manipulation of Purified DNA 55 5 Introduction of DNA into Living Cells 87 6 Cloning Vectors for Escherichia coli 105 7 Cloning Vectors for Eukaryotes 125 8 How to Obtain a Clone of a Specific Gene 151 9 The Polymerase Chain Reaction 175 Part II The Applications of Gene Cloning and DNA Analysis in Research 193 10 Sequencing Genes and Genomes 195 11 Studying Gene Expression and Function 221 12 Studying Genomes 245 13 Studying Transcriptomes and Proteomes 261 14 DNA Editing 279 Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 293 15 Production of Protein from Cloned Genes 295 16 Gene Cloning and DNA Analysis in Medicine 319 17 Gene Cloning and DNA Analysis in Agriculture 347 18 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 375 Glossary 393 Index 000

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Author Information

T. A. Brown is Emeritus Professor of Biomolecular Archaeology in the Department of Earth and Environmental Sciences at the University of Manchester in the United Kingdom. He has published several books on genetics, genomics, and biochemistry as well as over 150 research papers.

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