Overview

Full Product Details

Author:   Alan V. Smrcka
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   237
Dimensions:   Width: 15.50cm , Height: 1.50cm , Length: 23.50cm
Weight:   1.210kg
ISBN:  

9781588291370

ISBN 10:   1588291375
Pages:   250
Publication Date:   15 September 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Part I. Purification of G Protein Subunits and G Protein Effectors Purification of Recombinant G Protein a Subunits from Escherichia coli Wendy K. Greentree and Maurine E. Linder Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged a and bg Subunits Tohru Kozasa Expression and Purification of Soluble Adenylyl Cyclase from Eschericia coli Jeff A. Beeler and Wei-Jen Tang Purification of Phospholipase C b and Phospholipase C e from Sf9 Cells Mousumi Ghosh, Huan Wang, Grant G. Kelley, and Alan V. Smrcka Part II. Assays for G Protein-Dependent Effector Activation In Vitro Assay for G Protein-Dependent Activation of Phospholipase C b Using Purified Protein Components Mousumi Ghosh and Alan V. Smrcka Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells Ronald Taussig Part III. Assays for G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells Measurement of G Protein-Coupled Receptor-Stimulated Phospholipase D Activity in Intact Cells Stephanie J. Walker and H. Alex Brown Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells Bo Liu and Dianqing Wu Part IV. Receptor Mechanisms, Activity, and Localization Intensive Mutational Analysis of G Protein-Coupled Receptors in Yeast Andjelka ´Celi´c, Sara M. Connelly, Negin P. Martin, and Mark E. Dumont Green Fluorescent Protein-Tagged b-Arrestin Translocation as a Measure of G Protein-Coupled Receptor Activation Stephen S. G. Ferguson and Marc G. Caron Detection of G Protein-Coupled Receptors by Immunofluorescence Microscopy Patricia M. Hinkle and John A. Puskas Assay of G Protein-Coupled Receptor Activation of G Proteins in Native Cell Membranes Using [35S]GTPgS Binding Jean M. Bidlack and Amy L.Parkhill Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay Bo Liu and Dianqing Wu Part V. Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems Selective Inhibition of G Protein-Mediated Pathways Using RGS Domains Tohru Kozasa and Richard D. Ye Ribozymes as Tools for Suppression of G Protein g Subunits Janet D. Robishaw, Zheng-ping Guo, and Qin Wang In Vivo Adenoviral-Mediated Gene Transfer of the bARKct to Study the Role of Gbg in Arterial Restenosis Guido Iaccarino and Walter J. Koch Part VI. Posttranslational Modifications of Regulators of G Protein Signaling Analysis of RGS Protein Palmitoylation Leah S. Bernstein, Maurine E. Linder, and John R. Hepler Methods for Measuring RGS Protein Phosphorylation by G Protein-Regulated Kinases Susanne Hollinger and John R. Hepler Part VII. Use of GFP-Tagged G Proteins and Effectors in Intact Cells The Use of Green Fluorescent Proteins to View Association Between Phospholipase Cb and G Protein Subunits in Cells Suzanne Scarlata and Louisa Dowal Cellular Localization of GFP-Tagged a Subunits Thomas R. Hynes, Thomas E. Hughes, and Catherine H. Berlot Index

Reviews

"""...a timely and useful set of protocols...a significant contribution."" -Doody's Health Sciences Book Review Journal"

...a timely and useful set of protocols...a significant contribution. - Doody's Health Sciences Book Review Journal

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions