Overview

This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter  4 is available open access under a CC BY 4.0 license via link.springer.com.

Full Product Details

Author:   Mario Tiberi
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1947
Weight:   1.013kg
ISBN:  

9781493991204

ISBN 10:   1493991205
Pages:   406
Publication Date:   11 April 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Probing Structural Properties of GPCRs 1. Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics Chenyi Liao, Victor May, and Jianing Li 2. Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs Katsufumi Tomobe, Eiji Yamamoto, and Kenji Yasuoka 3. Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies Fabian Bumbak, Ross A.D. Bathgate, Daniel J. Scott, and Paul R. Gooley 4. A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties Anne Zemella, Theresa Richter, Lena Thoring, and Stefan Kubick Part II: Tweaking Ligands for GPCR Studies 5. Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions Marleen Van Troys, Willem Vannecke, Christophe Ampe, and Annemieke Madder 6. Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands Amanda Acosta-Ruiz, Johannes Broichhagen, and Joshua Levitz 7. Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs Sridevi M. Ramanoudjame, Lucie Esteoulle, Stephanie Riche, Jean-Francois Margathe, Thierry Durroux, Iuliia A. Karpenko, and Dominique Bonnet Part III: Bioluminescence and Fluorescence Resonance Transfer Energy Approaches 8. Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin, Dominique Bonnet, and Thierry Durroux 9. Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches Kyla Bourque, Dominic Devost, Asuka Inoue, and Terence E. Hebert 10. Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays James H. Felce, John R. James, and Simon J. Davis 11. Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions Amina M. Bagher, Melanie E.M. Kelly, and Eileen M. Denovan-Wright 12. Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters Andreas Koschinski and Manuela Zaccolo Part IV: Exploring GPCR Signaling Properties 13. Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays Genevieve Hamel-Cote, Fanny Lapointe, and Jana Stankova 14. Measurement of -Arrestin Recruitment at GPCRs Using the Tango Assay Genevieve Laroche and Patrick M. Giguere 15. Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry Burcu Hasdemir, Juan A. Oses-Prieto, Alma Burlingame, and Aditi Bhargava Part V: Visualizing Subcellular Compartmentalization of GPCRs 16. Monitoring the Aggregation of GPCRs by Fluorescence Microscopy Samuel Genier, Jade Degrandmaison, Christine L. Lavoie, Louis Gendron, and Jean-Luc Parent 17. Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies Xuezhi Li, Stephanie Rosciglione, Andreanne Laniel, and Christine Lavoie 18. Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy Fabiana A. Cateano-Crowley, Bryan Heit, and Stephen S.G. Ferguson 19. Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy Kim C. Jonas and Aylin C. Hanyaloglu Part VI: Investigating In Vivo Functions of GPCRs 20. Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug Marc Lopez-Cano, Joan Font, Amadeu Llebaria, Victor Fernandez-Duenas, and Francisco Ciruela 21. Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice Dmitry Akhmedov, Nicholas S. Kirkby, Jane A. Mitchell, and Rebecca Berdeaux 22. Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents Xavier Toussay, Mario Tiberi, and Baptiste Lacoste 23. Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs Martha Hinton, Anurag Singh Sikarwar, and Shyamala Dakshinamurti

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