Overview

This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

Full Product Details

Author:   Sofia Aires M. Martins ,  Duarte Miguel F. Prazeres
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2268
Weight:   0.662kg
ISBN:  

9781071612231

ISBN 10:   1071612239
Pages:   327
Publication Date:   06 June 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy             Peter Abdelmaseeh, Andrew C. Tiu, Selim Rozyyev, Laureano D. Asico, Pedro A. Jose, Van Anthony M. Villar   2. Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing             Krishna Sriram, Cristina Salmerón, Anna Di Nardo, and Paul A. Insel   3. Construction of Recombinant Cell Lines for GPCR Expression             Philip J. Reeves   4. Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor             Alexei A. Yeliseev   5. Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast             Emily A. Yasi and Pamela Peralta-Yahya   6. Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface             Jasmina Vidic and Yanxia Hou   7. Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples             Zoe Köck, Volker Dötsch, and Frank Bernhard   8. Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses             Santa Veiksina, Maris-Johanna Tahk, Tõnis Laasfeld, Reet Link, Sergei Kopanchuk, and Ago Rinken   9. Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology             Hannes Schihada, Katarina Nemec, Martin J. Lohse, and Isabella Maiellaro   10. Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction             Céline Laschet and Julien Hanson   11. Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity             Luca Bordes, Sergei Chavez-Abiega, and Joachim Goedhart   12. cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation             Darja Lavogina, Tõnis Laasfeld, Maris-Johanna Tahk, Olga Kukk, Anni Allikalt, Sergei Kopanchuk, and Ago Rinken   13. FLIPR Calcium Mobilization Assays in GPCR Drug Discovery             Grzegorz Woszczek, Elisabeth Fuerst, and Thomas J.A. Maguire   14. Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity             Xenia Meshik and N. Gautam   15. Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins             Marta Alonso-Gardón and Raúl Estévez   16. NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled Receptors             Xiaoyuan Ma, Rob Leurs, and Henry F. Vischer   17. Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias             Nicola C. Dijon, Desislava N. Nesheva, and Nicholas D. Holliday   18. Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber             Sara Kimiko Suzuki, Joshua B. Kelley, Timothy C. Elston, and Henrik G. Dohlman   19. Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays             Sofia Aires M. Martins, Duarte Miguel F. Prazeres, Virginia Chu, and João P. Conde   20. Homology Modeling Using GPCRM Web Service             Przemysław Miszta, Szymon Niewieczerzał, Paweł Pasznik, and Sławomir Filipek

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