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Author:   Fahd Al-Mulla
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   724
Weight:   0.839kg
ISBN:  

9781493961719

ISBN 10:   1493961713
Pages:   312
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research.-Tissue Microarrays: Construction and Uses.-Standardization in Immunohistology.-Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue.-Microwaves for Chromogenic-in-situ-hybridization.-Automated Analysis of FISH-stained HER2/neu Samples with Metafer.-Laser Capture Microdissection (LCM) of FFPE Tissue Sections Bridging the Gap between Microscopy and Molecular Analysis.-Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections.-Microarray-based CGH and Copy Number Analysis of FFPE Samples.-Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform.-Whole Genome Amplification of DNA Extracted From FFPE Tissues.-Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue.-Analysis of DNA Methylation in  FFPE Tissues using the MethyLight Technology.-RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions.-RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues.-microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues.-Gene Expression Profiling of RNA Extracted from FFPE tissues:  NuGEN Technologies’ Whole-Transcriptome Amplification System.-Protein Mass Spectrometry Applications on FFPE Tissue Sections.-An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2â-CS100 Approach            

Reviews

From the reviews: This text can be considered an introductory compendium of current methods and protocols in Molecular Biology for the use of Fixed Paraffin-Embedded Tissues that will be particularly suited to new researchers in the field who are eager to develop expertise. It is an easily readable and technically comprehensive book that would be ideal for initial experimental planning which can be complemented by other bibliographic sources and scientific journals for in-depth discussions of particular techniques and their potential applications. (Flavia Troglio and Manuela Vecchi, Molecular Biotechnology, 2012)

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