Overview

Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.

Full Product Details

Author:   Yuko Giga-Hama ,  Hiromichi Kumagai
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   Softcover reprint of the original 1st ed. 1997
Dimensions:   Width: 15.50cm , Height: 1.10cm , Length: 23.50cm
Weight:   0.308kg
ISBN:  

9783662034743

ISBN 10:   3662034743
Pages:   179
Publication Date:   18 April 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Fission Yeast Schizosaccharomycespombe: An Attractive Host for Heterologous Protein Production.- 2. Constitution of Expression Vectors.- 3. Exploiting the Thiamine Biosynthetic Gene nmtl for Regulated Expression of Heterologous DNAs in Fission Yeast.- 4. Development of Constitutive Expression Vector for the Highest Production Level of Heterologous Proteins in Fission Yeast Schizosaccharomycespombe.- 5. Overexpression of Proteins with Seven Transmembrane Spanning Segments.- 6. Targeting and Isolation of Tagged Membrane Proteins.- 7. Expression of a Human Cytochrome P450 Form in Schizosaccharomyces pombe: Comparison with Expression in Saccharomyces cerevisiae.- 8. Human Papillomavirus Capsid Protein-pREP in Schizosaccharomyces pombe: Efficient Assembly of the Viral Capsid Protein in S. pombe and S. cerevisiae.- 9. Expression, Purification, and Analyses of Cell-Cycle Regulatory Proteins in S. pombe.- 10. Expression and Secretion of Mammalian Proteins in Schizosaccharomyces pombe.- 11. Secretion of Human Interleukin-6 Using the P-Factor Secretion Signal in Schizosaccharomyces pombe.- Color Figures.

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