Overview

This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.Contents: Fluorescence Lifetime Imaging by Double Pulse Excitation in Bilateral Confocal Microscopy (G J Brakenhoff et al.) Icosahedral Virus Structure Determination by Electron Cryomicroscopy: Image Processing Principles (P A Thuman-Commike & W Chiu) Appropriate Image Processing for Confocal Microscopy (G Cox & C J R Sheppard) X-Ray Fluorescence Two-Dimensional Microanalysis at the VEPP-3 Storage Ring: Applications in Environmental Science (I P Dolbnya & K V Zolotarev) 3D Transfer-Function Analysis for 4Pi Confocal Microscopy (M Gu & C J R Sheppard) Image Restoration of Thick Biological Specimens for Transmission Electron Microscope Tomography (K F Han et al.) Images of Membrane-Bound Detoxification Enzyme at 4A Resolution Obtained by Electron Cryomicroscopy (I Schmidt-Krey et al.) The Uptake Pathway of DNA and Lipids in Cationic Liposome-Mediated Gene Transfer (S-W Hui et al.) Multiscale Imaging, Analysis and Modeling of the Pulmonary Bronchial Tree (A Kriete et al.) Asymmetric Cell Division as a Mechanism of Cell Determination in Vascular Plants (B-L Lin) 3D Electron Microscopy of Cells and Organelles by High-Voltage EM, Tomography and Stereopair Analysis (M Marko) Surface Scattering Techniques in Scanning Optical Microscopy (J F Aguilar & E R MEndez) Applications of Electron Microscopy to Materials and Earth Sciences - Dislocation, Shape and Orientation Changes and Interdiffusion (P Shen) Surface Profiling and Confocal Microscopy (C J R Sheppard & J C Quartel) Matched Spatial Filters in Long Working Distance Microscopy of Phase Objects (J R Strickler & J-S Hwang) Transport Pathways of Lipoproteins Across the Arterial Endothelial Cells (C-H Kao et al.) Polarisation Effects in Confocal Microscopy (T Wilson) Near-Field Microscopy and Spectroscopy of Single Molecules, Single Proteins and Biological Membranes (X S Xie et al.)

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