Overview

This unique text offers a systematic and practical approach to the analysis and interpretation of FCM graphics. Using numerous FCM illustrations derived from actual well-documented clinical cases, the authors demonstrate a step-by-step approach to optimal FCM data analysis on specimens suspected of harboring hematopoietic malignancies. The discussion moves from simple to complex specimens, with an emphasis on visual pattern analysis. A wide variety of hematologic disorders are covered, including leukemias and lymphomas. The companion CD-ROM with 80 detailed case studies provides additional opportunities to gain a deeper understanding of FCM data analysis.

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9781588292124

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Reviews

Wow! This is really a nice book. There are only five chapters, but be forewarned - each chapter is quite comprehensive in its discussion of the subject matter. The text is factually dense and thorough. The opening two chapters logically and concisely lay out overall considerations for immunophenotyping -- the theoretical underpinnings, strengths and weaknesses of the technology, specimen considerations, how to decide what should be components of antibody panels, etc. I especially liked the brief but succinct and cautionary discussion of the lineage infidelity and limited clinical utility of various diagnostic cell surface markers. The third chapter eases the reader into the easiest category of studies to interpret - i.e., homogeneous specimens. The final two chapters delve into great depth on the analysis of heterogeneous specimens - those containing both normal and abnormal populations -- commonly encountered in the clinical setting and posing the greatest interpretative challenge. This is a really great book! It thoroughly summarizes all aspects of flow cytometry in the practice of hematopathology. I'm considering making it required reading for my residents. - Doody's Health Sciences Book Review Journal

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