Overview

Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Full Product Details

Author:   Michael Ormerod (Scientific Consultant, Scientific Consultant)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   3rd Revised edition
Volume:   229
Dimensions:   Width: 18.80cm , Height: 1.70cm , Length: 24.50cm
Weight:   0.580kg
ISBN:  

9780199638246

ISBN 10:   0199638241
Pages:   296
Publication Date:   18 May 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Introduction to the principles of flow cytometry ; Fluorescence and fluorescence technology ; Preparing of suspensions of single cells ; Flow sorting ; Immunofluorescence of surface markers ; Analysis of DNA - general methods ; Further clinical applications ; Quality control in the clinical laboratory ; Measurement of cytoplasmic and nuclear antigens ; Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method ; Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method ; Chromosome analysis and sorting by flow cytometry ; Intracellular ionised calcium, magnesium, membrane potential and pH ; Flow cytometry in the study of apoptosis ; Further applications to cell and molecular biology

Reviews

This book will be useful to those people who wish to begin exploring the fascinating and attractive world of flow cytometry and also to those who need to update their knowledge of flow cytometry protocols in mammalian cell biology. It is also an excellent reference book on flow cytometry for use in the laboratory. Cell Biology International

This book will be useful to those people who wish to begin exploring the fascinating and attractive world of flow cytometry and also to those who need to update their knowledge of flow cytometry protocols in mammalian cell biology. It is also an excellent reference book on flow cytometry for use in the laboratory. Cell Biology International

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