Overview

This authored book is focused on SDG 14: Life below water, comprehensively addressing all facets of biotechnology and bioinformatics related to fisheries. It offers an extensive exploration of the detail on structure, function and types of nucleic acids, concepts of gene and genetic code, mutations, and their implications. The book provides essential information on gene regulation and expression in prokaryotes and eukaryotes. Step-by-step descriptions are provided for technologies such as gene transfer, rDNA, transgenic fish production, animal cell culture, hybridoma technology and cryopreservation technology in fishes. Special emphasis has been given to topics like RNA in gene regulation, epigenetics, and DNA and protein sequencing. Various molecular techniques and markers have been discussed in detail. Further, various topics on bioinformatics including different databases, formats, sequence retrieval, manipulation, analysis, primer design, molecular visualization, genomics, and proteomics are also covered. This volume will prove invaluable to aquaculturists, equipping them with essential techniques and protocols. It constitutes essential reading for students enrolled in aquaculture or fisheries courses within tropical and sub-tropical regions.

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9789819969906

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Judith Betsy is an Assistant Professor at the Department of Aquaculture, Fisheries College and Research Institute of Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Tuticorin, India. Holding a doctorate in Aquaculture, she is a recipient of the Maulana Azad National Fellowship from the University Grants Commission, New Delhi, India. Having worked in fish gamete cryopreservation and spermatology for the past 10 years, she has been honoured with 10 awards including gold medals for her outstanding academic performance during her graduate studies and has published over 30 research papers in peer-reviewed journals around the world, more than 85 technical articles, 6 book chapters and 6 books.   Siva is a Scientist at the Indian Council of Agricultural Research - Directorate of Coldwater Fisheries Research, Bhimtal, India. He has more than eight years of experience in the field of Fish Genetics and Breeding and has operated around ten institutionally and externally funded research projects in various capacities. He has been involved in developing complete mitogenomes, transcriptome assemblies, and molecular markers for important coldwater fishes of India. He has been involved in studies related to the identification of different fish genetic stocks in India and mining genes and alleles related to economic traits and sex-related functions in fish. He has several research publications to his credit. At present, he is working in the area of comparative transcriptomics to study the underlying genetic mechanisms of sex determination and differentiation in important coldwater fish species of India.

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