Overview

DNA and RNA fingerprinting based on arbitrarily primed PCR (polymerase chain reaction) provides a powerful tool for the study of genes. The basic techniques are described here in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow the user to choose between alternative strategies. In addition to the general techniques, specific research applications of particular interest are given, such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumours or differentially expressed genes by RNA fingerprinting.

Full Product Details

Author:   M. R. Micheli ,  Rodolfo Bova
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Dimensions:   Width: 19.30cm , Height: 2.00cm , Length: 24.20cm
Weight:   0.965kg
ISBN:  

9783540612292

ISBN 10:   3540612297
Pages:   457
Publication Date:   30 November 1996
Audience:   Professional and scholarly ,  General/trade ,  Professional & Vocational
Format:   Book
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Overview.- I DNA Extraction from Mammals.- II Insect DNA Extraction Protocol.- III Rapid DNA Extraction from Plants.- IV Preparation of Fungal Genomic DNA for PCR and RAPD Analysis.- V Extraction of Histoplasma capsulatum DNA for PCR.- VI DNA Extraction from Bacterial Cultures.- VII Arbitrarily Primed PCR and RAPDs.- VIII Random Amplified Polymorphic DNA Assay.- IX DNA Amplification Fingerprinting.- X Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers.- XI Optimization of RAPD Fingerprinting.- XII Fingerprint Tailoring.- XIII Resolving DNA Amplification Products Using Polyacrylamide Gel Electrophoresis and Silver Staining.- XIV Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms.- XV Modified Temperature Sweep Gel Electrophoresis for the Separation of Arbitrarily Amplified DNA Fragments.- XVI High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization.- XVII Recovering Amplified DNA from Silver Stained Gels.- XVIII Cloning of RAPD Markers.- XIX Sequencing of RAPD Markers.- XX Analysis of Tumor-Specific Genetic Alterations by Arbitrarily Primed PCR.- XXI Construction of Linkage Maps with RAPD Markers.- XXII Pseudo-Testcross Mapping Strategy Using RAPD Markers.- XXIII Estimating Nucleotide Divergence with RAPD Data.- XXIV RAPD and PAUP Analysis for Microbial Screening Programs.- XXV Production of Specific Probes for Microorganisms.- Overview.- XXVI Differential Display of Expressed mRNAs.- XXVII RNA Arbitrarily Primed PCR.- XXVIII Nonradioactive Differential Display of Messenger RNA.- XXIX Fluorescent Differential Display: A Fast and Safe Way for Reliable Differential Display Analysis.- XXX Slot Blot Hybridization Screening.- XXXI How To Find and Clone the Appropriate cDNA Fragments Generated in Differential mRNA Display by Using Northern Blot for cDNA Capture.- XXXII Verification of Differential Display Results by RNase Protection.- XXXIII Direct Automated Sequencing of DDRT-PCR Fragments.- XXXIV Ligation Linked PCR and Direct Sequencing of Differential Display Products.- XXXV Differential Display PCR Fragments as Probes for cDNA Cloning.- XXXVI Targeted RNA Fingerprinting.- XXXVII Generation of Tumor-Specific DEST Catalogue.- XXXVIII Analysis of Gene Expression in the Preimplantation Mouse Embryo Using mRNA Differential Display.- XXXIX Rapid Identification of Differentially Expressed Genes in Trypanosomes.

Reviews

The only primer you'll ever need... great care has been taken in putting this book together, and the results are very impressive. Although the work of many authors, the contributions form a coherent and well-organized whole. Trends in Genetics ...buy this excellent book. Cellular and Molecular Biology

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