Overview

This detailed volume explores fibrous proteins widely present in different biological tissues or biological structural materials. The book begins by introducing the structure of representative fibrous proteins, including animal silks, collagen, elastin, resilin, and keratin, and it then continues by providing detailed experimental protocols for the synthesis, assembly, and characterization of natural, regenerated, and recombinant fibrous proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Fibrous Proteins: Design, Synthesis, and Assembly is an ideal guide for researchers aiming to master fibrous protein preparations with the aid of this broad and interdisciplinary perspective on understanding the structure-property-function relationships of natural and reconstituted fibrous proteins.

Full Product Details

Author:   Shengjie Ling
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2347
Weight:   0.529kg
ISBN:  

9781071615768

ISBN 10:   1071615769
Pages:   264
Publication Date:   03 September 2022
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Introduction to Fibrous Proteins   1. The Structure of Animal Silks             Wenwen Zhang and Yimin Fan   2. The Structure of Collagen             Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren   3. The Structure of Elastin             Yuelong Xiao, Shengjie Ling, and Ying Pei   4. The Structure of Resilin             Yuelong Xiao, Shengjie Ling, and Ying Pei   5. The Structure of Keratin             Wenwen Zhang and Yimin Fan   Part II: Methods in Synthesis of Fibrous Proteins   6. General Methods to Produce and Assemble Recombinant Spider Silk Proteins             Na Kong   7. Self-Assembly of Bombyx mori Silk Fibroin             Na Kong   8. Synthesis and Assembly of Recombinant Collagen             Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren   9. Recombination and Purification of Elastin-Like Polypeptides             Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren   10. Methods to Synthesize and Assemble Recombinant Keratins             Wenwen Zhang and Yimin Fan   11. Preparation of Amyloid Fibers Using Recombinant Technology             Wenwen Zhang and Yimin Fan   12. Molecular Biology Methods to Construct Recombinant Fibrous Protein             Jicong Zhang   Part III: Methods in Assembly of Fibrous Proteins   13. Isolation of Nanofibrils from Animal Silks             Ke Zheng   14. Gelation Methods to Assemble Fibrous Proteins             Ning Fan and Ke Zheng   15. Spinning Methods Used for The Construction of One- and Two-Dimensional Fibrous Protein Materials             Leitao Cao   16. Three-Dimensional Printing to Build Fibrous Protein Architectures             Huanhuan Qiao and Ke Zheng   Part IV: Methods in Characterization of Fibrous Proteins   17. The Methods of Using Synchrotron FTIR Microspectroscopy to Understand the Conformation of Single Animal Silk Fibers             Chao Ye, Leitao Cao, and Shengjie Ling   18. The Protocol of Using FTIR Imaging to Investigate Silk Fibroin-Based Materials             Jiajia Zhong, Xiaojie Zhou, Chao Ye, Wenjie Yu, and Yuzhao Tang   19. Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy             Yawen Liu, Hongchong Guo, and Shengjie Ling   20. The Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins             Wenli Gao, Ting Shu, Qiang Liu, Hongchong Guo, Shengjie Ling, and Liang Zhou   21. Structural Characterization of Silk Fibers by Wide-Angle X-Ray Scattering             Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling   22. Structural Characterization of Silk Fibers by Small-Angle X-Ray Scattering             Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling

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