Overview

This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Full Product Details

Author:   Miquel Vila-Perelló
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2133
Weight:   0.706kg
ISBN:  

9781071604366

ISBN 10:   1071604368
Pages:   362
Publication Date:   07 March 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Methods and Applications of Expressed Protein Ligation Zhipeng A. Wang and Philip A. Cole   2. Protein Splicing: From the Foundations to the Development of Biotechnological Applications Alejandro Romero-Casañas, Verónica Gordo, Jessica Castro, and Marc Ribó   3. Identification, Characterization, and Optimization of Split Inteins Neel H. Shah and Adam J. Stevens   4. Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins Jing Zhao, Zhenming Du, Chunyu Wang, and Kenneth V. Mills   5. Expressed Protein Ligation: General Experimental Protocols Joshua Diaz  and Jean-Philippe Pellois   6. Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins Yu Wang and Yi-Ming Li   7. Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis Judith Palà-Pujadas  and Juan B. Blanco-Canosa   8. Fused Split Inteins: Tools for Introducing Multiple Protein Modifications Byung Joon Lim, Raymond F. Berkeley, and Galia T. Debelouchina   9. Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate Silvia Frutos   10. In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins Nicholas A. Prescott and Yael David   11. Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation Benjamin J. Umlauf and Eric V. Shusta   12. Site-specific Phosphorylation of PDZ Domains Sana Ma, Kristian Strømgaard and Louise S. Clemmensen   13. Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications Nora Guidotti and Beat Fierz   14. Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol Caroline E. Weller and Champak Chatterjee   15. Investigating the Effects of O-GlcNAc Modifications in Parkinson’s Disease using Semi-synthetic α-synuclein Ana Galesic and Matthew R. Pratt   16. Recombinant Expression of Cyclotides Using Expressed Protein Ligation Maria Jose Campbell, Jingtan Su, and Julio A. Camarero   17. Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines Somnath Mukherjee, Maria Matveenko, and Christian F.W. Becker  

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions