Overview

This detailed book focuses on cutting-edge methods for Parkinson’s disease (PD) research, such as the analysis of the prion-like properties of α-synuclein, mitochondrial functions related to the PINK1-Parkin pathway/CHCHD2, the endolysosome pathway related to LRRK2, VPS35, and ATP13A2 using cultured cells (including patient iPS cells), deep brain stimulation therapy, classic mitochondrial toxins related to PD, and genetic associations and screenings using mammalian and invertebrate genetic models of PD. The collection intends to serve as an introductory protocol book for basic research on PD pathogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Experimental Models of Parkinson's Disease is an ideal guide for researchers continuing to work toward making PD risk predictable and surmountable.

Full Product Details

Author:   Yuzuru Imai
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2322
Weight:   0.448kg
ISBN:  

9781071614976

ISBN 10:   1071614975
Pages:   217
Publication Date:   29 May 2022
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Biochemical Experiments and Cellular Models of Parkinson’s Disease   1. α-Synuclein Seeding Assay Using RT-QuIC             Ayami Okuzumi, Taku Hatano, Takeshi Fukuhara, Shinichi Ueno, Nobuyuki Nukina, Yuzuru Imai, and Nobutaka Hattori   2. Electron-Microscopic Analysis of α-Synuclein Fibrils             Airi Tarutani and Masato Hasegawa   3. α-Synuclein Seeding Assay Using Cultured Cells             Jun Ogata, Daisaku Takemoto, Shotaro Shimonaka, Yuzuru Imai, and Nobutaka Hattori   4. Analysis of α-Synuclein in Exosomes             Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, and Nobutaka Hattori   5. Measurement of GCase Activity in Cultured Cells             Yuri Shojima, Jun Ogata, Taiji Tsunemi, Yuzuru Imai, and Nobutaka Hattori   6. Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells             Kyohei Ito, Lejia Xu, Genta Ito, and Taisuke Tomita   7. Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement             Maria Sakurai and Tomoki Kuwahara   8. Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells             Kei-ichi Ishikawa, Risa Nonaka, and Wado Akamatsu   9. Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells             Kahori Shiba-Fukushima and Yuzuru Imai   Part II: Mammalian Models of Parkinson’s Disease   10. Generation of Mitochondrial Toxin Rodent Models of Parkinson’s Disease Using 6-OHDA, MPTP, and Rotenone             Hiroharu Maegawa and Hitoshi Niwa   11. Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease             Yuji Kamikubo, Keiko Wakisaka, Yuzuru Imai, and Takashi Sakurai   12. α-Synuclein Propagation Mouse Models of Parkinson’s Disease             Norihito Uemura, Jun Ueda, Shinya Okuda, Masanori Sawamura, and Ryosuke Takahashi   13. Common Marmoset Model of α-Synuclein Propagation             Masami Masuda-Suzukake, Aki Shimozawa, Masashi Hashimoto, and Masato Hasegawa   14. Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections             Kenta Yamauchi, Megumu Takahashi, and Hiroyuki Hioki   15. Deep Brain Stimulation Using Animal Models of Parkinson’s Disease             Asuka Nakajima and Yasushi Shimo   Part III: Invertebrate Models of Parkinson’s Disease   16. Assessment of Cytotoxicity of α-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy             Masak Takaine   17. The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons             Tomoki Kuwahara   18. Analysis of Dopaminergic Functions in Drosophila             Tsuyoshi Inoshita, Daisaku Takemoto, and Yuzuru Imai   19. Evaluation of Mitochondrial Function and Morphology in Drosophila             Yinglu Tang, Foozhan Tahmasebinia, and Zhihao Wu   20. Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons             Tsuyoshi Inoshita and Yuzuru Imai

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