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Author:   Joyce A. Lloyd
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1698
Weight:   7.318kg
ISBN:  

9781493974276

ISBN 10:   1493974270
Pages:   298
Publication Date:   27 October 2017
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

An Introduction to Erythropoiesis Approaches.- Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis.- Mouse Models of Erythropoiesis and Associated Diseases.- Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells.- Stress Erythropoiesis Model Systems.- Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models.- Functional Analysis of Erythroid Progenitors by Colony-Forming Assays.- Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands.- Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells.- Analysis of Erythropoiesis Using Imaging Flow Cytometry.- Flow Cytometric Analysis of Erythroblast Enucleation.- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure.- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples.- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples.- Genome Editing of Erythroid Cell Culture Model Systems.- In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood.- Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.- Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products.

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