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Author:   Joanna B. Wilson ,  Gerhard H. W. May
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   174
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.50cm
Weight:   2.400kg
ISBN:  

9780896036901

ISBN 10:   0896036901
Pages:   438
Publication Date:   23 March 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Genome and Transcript Analyses.- Epstein-Barr Virus.- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR.- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids.- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs).- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells.- Visualizing EBV Expression Patterns by FISH.- Viral Detection.- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products.- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections.- Detection of EBV Infection at the Single-Cell Level.- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo.- Culture Methods.- Virus Isolation.- Generation of Lymphoblastoid Cell Lines (LCLs).- Cell Cycle Distribution of B-Lymphocytes and Cell Lines.- of Plasmid Vectors into Cells Via Electroporation.- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene.- Transient Gene Expression and MACS Enrichment.- In Vitro Assays to Study Epithelial Cell Growth.- In Vitro Assays to Study Epithelial Cell Differentiation.- Cell Sensitivity Assays.- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation.- Immune Assays.- Regression Assay.- Generation of Polyclonal EBV-Specific CTL Cultures and Clones.- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs.- Limiting Dilution Assay.- Viral Protein Detection.- Antibodies for Detecting EBV Latent Proteins.- Detection of EBV Latent Proteins by Western Blotting.- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis.- Protein-Protein, Protein-DNA, and Protein-RNA Interactions.- The Yeast Two-Hybrid Assay to Identify Interacting Proteins.- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins.- Magnetic DNA Affinity Purification of a Cellular Transcription Factor.- Screening an Expression Library with a DNA Binding Site.- Analysis of DNA Binding Proteins by Mobility Shift Assay.- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs.- Protein Activity Assays.- Chimeric and Mutated Variants of LMP1.- Assaying the Activity of Kinases Regulated by LMP1.- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities.- Tissue and In Vivo Protocols.- Analysis of Apoptosis in Tissue Sections.- Considerations in Generating Transgenic Mice.- In Vivo Assay of Cellular Proliferation.- Topical Chemical Carcinogen Treatment in Mice.- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture.- Selection and Enrichment of B Cells from Lymphoid Tissues.- Detection of Immunoglobulin Gene Rearrangements.

Reviews

"""Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer."" - Anticancer Research ""Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter."" -Today's Life Science ""This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..."" ""Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology."" -Microbiology Today ""Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design."" -Tumori ""This book is highly recommended for collections serving researchers in the area of molecular biology."" -E-Streams"

Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer. - Anticancer Research Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter. - Today's Life Science This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive... Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology. - Microbiology Today Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design. - Tumori

Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer. - Anticancer Research Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter. -Today's Life Science This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive... Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology. -Microbiology Today Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design. -Tumori This book is highly recommended for collections serving researchers in the area of molecular biology. -E-Streams

Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer. - Anticancer Research Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter. -Today's Life Science This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive... Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology. -Microbiology Today Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design. -Tumori This book is highly recommended for collections serving researchers in the area of molecular biology. -E-Streams

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