Overview

Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols.

Full Product Details

Author:   Michael M. Burrell
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1993 ed.
Volume:   16
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   1.190kg
ISBN:  

9780896033221

ISBN 10:   0896033228
Pages:   370
Publication Date:   22 May 1993
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Nucleases.- Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1).- DNA Polymerases (EC 2.7.7.7).- Taq Polymerase (EC 2.7.7.7).- Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6).- Reverse Transcriptase (EC 2.7.7.49).- Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31).- Restriction Enzymes.- DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73).- DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3).- The BAL 31 Nucleases (EC 3.1.11).- Mung-Bean Nuclease 1 (EC 3.1.30.1).- RNase A (EC 3.1.27.5).- Pronase (EC 3.4.24.4).- Proteolytic Enzymes for Peptide Production.- Proteinase K (EC 3.4.21.14).- Carboxypeptidase Y (EC 3.4.16.1).- Aminopeptidases.- Alkaline Phosphatase (EC 3.1.3.1).- Polynucleotide Kinase (EC 2.7.1.78).

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An essential resource for every biochemist and scientist in molecular biology. - Unlisted Drugs

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