Overview

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

Full Product Details

Author:   T Palmer ,  P L Bonner (Nottingham Trent University, UK)
Publisher:   Elsevier Science & Technology
Imprint:   Horwood Publishing Ltd
Edition:   2nd edition
Dimensions:   Width: 15.60cm , Height: 2.40cm , Length: 23.40cm
Weight:   0.650kg
ISBN:  

9781904275275

ISBN 10:   1904275273
Pages:   432
Publication Date:   04 April 2007
Audience:   College/higher education ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Authors' PrefacePart 1: Structure and Function of Enzymes 1: An Introduction to Enzymes 1.1 WHAT ARE ENZYMES?1.2 A BRIEF HISTORY OF ENZYMES1.3 THE NAMING AND CLASSIFICATION OF ENZYMESSUMMARY OF CHAPTER 1PROBLEMS2: The Structure of Proteins 2.1 INTRODUCTION2.2 AMINO ACIDS, THE BUILDING BLOCKS OF PROTEINS2.3 THE BASIS OF PROTEIN STRUCTURE2.4 THE DETERMINATION OF PRIMARY STRUCTURE2.5 THE DETERMINATION OF PROTEIN STRUCTURE BY X-RAY CRYSTALLOGRAPHY2.6 THE INVESTIGATION OF PROTEIN STRUCTURE IN SOLUTIONSUMMARY OF CHAPTER 2PROBLEMS3: The Biosynthesis and Properties of Proteins 3.1 THE BIOSYNTHESIS OF PROTEINS3.2 THE PROPERTIES OF PROTEINSSUMMARY OF CHAPTER 3PROBLEMS4: Specificity of Enzyme Action 4.1 TYPES OF SPECIFICITY4.2 THE ACTIVE SITE4.3 THE FISCHER `LOCK-AND-KEY' HYPOTHESIS4.4 THE KOSHLAND `INDUCED-FIT' HYPOTHESIS4.5 HYPOTHESES INVOLVING STRAIN OR TRANSITION-STATE STABILIZATION4.6 FURTHER COMMENTS ON SPECIFICITYSUMMARY OF CHAPTER 45: Monomeric and Oligomeric Enzymes 5.1 MONOMERIC ENZYMES5.2 OLIGOMERIC ENZYMESSUMMARY OF CHAPTER 5Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions 6: An Introduction to Bioenergetics, Catalysis and Kinetics 6.1 SOME CONCEPTS OF BIOENERGETICS6.2 FACTORS AFFECTING THE RATES OF CHEMICAL REACTIONS6.3 KINETICS OF UNCATALYSED CHEMICAL REACTIONS6.4 KINETICS OF ENZYME-CATALYSED REACTIONS: AN HISTORICAL INTRODUCTION6.5 METHODS USED FOR INVESTIGATING THE KINETICS OF ENZYME-CATALYSED REACTIONS6.6 THE NATURE OF ENZYME CATALYSISSUMMARY OF CHAPTER 6PROBLEMS7: Kinetics of Single-Substrate Enzyme-Catalysed Reactions 7.1 THE RELATIONSHIP BETWEEN INITIAL VELOCITY AND SUBSTRATE CONCENTRATION7.2 RAPID-REACTION kINETICS7.3 THE kING AND ALTMAN PROCEDURESUMMARY OF CHAPTER 7PROBLEMS8: Enzyme Inhibition 8.1 INTRODUCTION8.2 REVERSIBLE INHIBITION8.3 IRREVERSIBLE INHIBITIONSUMMARY OF CHAPTER 8PROBLEMS9: Kinetics of Multi-Substrate Enzyme-Catalysed Reactions 9.1 EXAMPLES OF POSSIBLE MECHANISMS9.2 STEADY-STATE KINETICS9.3 INVESTIGATION OF REACTION MECHANISMS USING STEADY-STATE METHODS9.4 INVESTIGATION OF REACTION MECHANISMS USING NON-STEADY-STATE METHODSSUMMARY OF CHAPTER 9PROBLEMS10: The Investigation of Active Site Structure 10.1 THE IDENTIFICATION OF BINDING SITES AND CATALYTIC SITES10.2 THE INVESTIGATION OF THE THREE-DIMENSIONAL STRUCTURES OF ACTIVE SITESSUMMARY OF CHAPTER 10PROBLEM11: The chemical nature of enzyme catalysis 11.1 AN INTRODUCTION TO REACTION MECHANISMS IN ORGANIC CHEMISTRY11.2 MECHANISMS OF CATALYSIS11.3 MECHANISMS OF REACTIONS CATALYSED BY ENZYMES WITHOUT COFACTORS11.4 METAL-ACTIVATED ENZYMES AND METALLOENZYMES11.5 THE INVOLVEMENT OF COENZYMES IN ENZYME-CATALYSED REACTIONSSUMMARY OF CHAPTER 1112: The Binding of Ligands to Proteins 12.1 INTRODUCTION12.2 THE BINDING OF A LIGAND TO A PROTEIN HAVING A SINGLE LIGAND-BINDING SITE12.3 COOPERATIVITY12.4 POSITIVE HOMOTROPIC COOPERATIVITY AND THE HILL EQUATION12.5 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING TWO BINDING SITES FOR THAT LIGAND12.6 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING THREE BINDING SITES FOR THAT LIGAND12.7 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING FOUR BINDING SITES FOR THAT LIGAND12.8 INVESTIGATION OF COOPERATIVE EFFECTS12.9 THE BINDING OF OXYGEN TO HAEMOGLOBINSUMMARY OF CHAPTER 12PROBLEMS13: Sigmoidal Kinetics and Allosteric Enzymes 13.1 INTRODUCTION13.2 THE MONOD-WYMAN-CHANGEUX (MWC) MODEL13.3 THE KOSHLAND-NEMETHY-FILMER (KNF) MODEL13.4 DIFFERENTIATION BETWEEN MODELS FOR COOPERATIVE BINDING IN PROTEINS13.5 SIGMOIDAL KINETICS IN THE ABSENCE OF COOPERATIVE BINDINGSUMMARY OF CHAPTER 13PROBLEMS14: The Significance of Sigmoidal Behaviour 14.1 THE PHYSIOLOGICAL IMPORTANCE OF COOPERATIVE OXYGEN-BINDING BY HÎ EMOGLOBIN14.2 ALLOSTERIC ENZYMES AND METABOLIC REGULATIONSUMMARY OF CHAPTER 14Part 3: Application of Enzymology 15: Investigation of Enzymes in Biological Preparations 15.1 CHOICE OF PREPARATION FOR THE INVESTIGATION OF ENZYME CHARACTERISTICS15.2 ENZYME ASSAY15.3 INVESTIGATION OF SUB-CELLULAR COMPARTMENTATION OF ENZYMESSUMMARY OF CHAPTER 15PROBLEM16: Extraction and Purification of Enzymes 16.1 EXTRACTION OF ENZYMES16.2 PURIFICATION OF ENZYMES16.3 DETERMINATION OF MOLECULAR WEIGHTS OF ENZYMESSUMMARY OF CHAPTER 16PROBLEM17: Enzymes as Analytical Reagents 17.1 THE VALUE OF ENZYMES AS ANALYTICAL REAGENTS17.2 PRINCIPLES OF ENZYMATIC ANALYSIS17.3 HANDLING ENZYMES AND COENZYMESSUMMARY OF CHAPTER 17PROBLEMS18: Instrumental Techniques Available for Use in Enzymatic Analysis 18.1 PRINCIPLES OF THE AVAILABLE DETECTION TECHNIQUES18.2 AUTOMATION IN ENZYMATIC ANALYSIS18.3 HIGH-THROUGHPUT ASSAYS (HTA)SUMMARY OF CHAPTER 1819: Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry 19.1 APPLICATIONS IN MEDICINE19.2 APPLICATIONS IN FORENSIC SCIENCE19.3 APPLICATIONS IN INDUSTRYSUMMARY OF CHAPTER 1920: Biotechnological Applications of Enzymes 20.1 LARGE-SCALE PRODUCTION AND PURIFICATION OF ENZYMES20.2 IMMOBILIZED ENZYMES20.3 ENZYME UTILIZATION IN INDUSTRYSUMMARY OF CHAPTER 20PROBLEMS21: Genomics, Proteomics and Bioinformatics 21.1 ENZYMES AND RECOMBINANT DNA TECHNOLOGY21.2 PROTEOMICS21.3 ENZYMES AND BIOINFORMATICSSUMMARY OF CHAPTER 21PROBLEMSAnswers to ProblemsAbbreviationsIndex

Reviews

Essential. ...explains the structure and function of enzymes. ...every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book., (Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK) Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine., (Review of the first edition) Natural Products Report (Royal Society of Chemistry) An excellent book, warmly recommended., (Review of the first edition) Trends in Biotechnology

Essential. ...explains the structure and function of enzymes. ...every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book., (Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK)Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine., (Review of the first edition) Natural Products Report (Royal Society of Chemistry)An excellent book, warmly recommended., (Review of the first edition) Trends in Biotechnology

Essential. ...explains the structure and function of enzymes. .every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book., (Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK) Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine., (Review of the first edition) Natural Products Report (Royal Society of Chemistry) An excellent book, warmly recommended., (Review of the first edition) Trends in Biotechnology

Essential. ...explains the structure and function of enzymes. ...every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book., (Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK) Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine., (Review of the first edition) Natural Products Report (Royal Society of Chemistry) An excellent book, warmly recommended., (Review of the first edition) Trends in Biotechnology

Author Information

Trevor Palmer and Philip L Bonner, Nottingham Trent University, UK

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