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Author:   Dan S. Tawfik (Principal Investigator, Department of Biomolecular Sciences, Weizmann Institute of Science, Israel)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.610kg
ISBN:  

9780128244319

ISBN 10:   0128244313
Pages:   312
Publication Date:   16 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Engineering lytic polysaccharide monooxygenases (LPMOs) Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink 2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases Daniela M. Carminati, Eric J. Moore and Rudi Fasan 3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer 4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution Duncan S. Macdonald and Donald Hilvert 5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier 6. Engineering aldolases for asymmetric synthesis Mikael Widersten 7. Ultra-high throughput screening for novel protease specificities Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke 8. Directed evolution of transcriptional switches using dual-selector systems Yuki Kimura and Daisuke Umeno 9. Methods for competitive enrichment and evaluation of superior DNA ligases Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley 10. Directed evolution of custom polymerases using droplet microfluidics Derek Vallejo, Ali Nikoomanzar and John C. Chaput 11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution Pu Xue, Tong Si and Huimin Zhao 12. Split enzymes: Design principles and strategy Shion A. Lim and James A. Wells

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Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

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