Overview

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Full Product Details

Author:   Robert Eisenthal (Department of Biology and Biochemistry, University of Bath, UK) ,  Michael Danson (Department of Biology and Biochemistry, University of Bath, UK)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   257
Dimensions:   Width: 19.60cm , Height: 2.20cm , Length: 25.50cm
Weight:   0.774kg
ISBN:  

9780199638215

ISBN 10:   0199638217
Pages:   302
Publication Date:   11 April 2002
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

1: Keith F Tipton: Principles of enzyme assay and kinetic studies 2: Robert A John: Photometric assays 3: Kelvin T Hughes: Radiometric assays 4: Shabih E H Syed: High performance liquid chromatography assays 5: J B Clark: Electrochemical assays: the oxygen electrode 6: R D Hurst and J B Clark: Electrochemical assays: the nitric oxide electrode 7: Keith Brocklehurst: Electrochemical assays: the pH-stat 8: Gunter M Rothe: Enzyme assays after gel electrophoresis 9: Nicholas C Price and Lewis Stevens: Techniques for enzyme extraction 10: Mark T Martin: Determination of active site concentration 11: David Hayes and Geoff Mellor: High throughput screening - considerations for enzyme assays 12: Athel Cornish-Bowden: Statistical analysis of enzyme kinetic data

Reviews

Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. Natural Product Reports

Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. * Natural Product Reports *

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