Overview
Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.
Full Product Details
Author: Nicole I. Nieden
Publisher: Humana Press Inc.
Imprint: Humana Press Inc.
Edition: Softcover reprint of the original 1st ed. 2011
Volume: 690
Weight: 0.899kg
ISBN: 9781493957231
ISBN 10: 1493957236
Pages: 336
Publication Date: 23 August 2016
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: Manufactured on demand
We will order this item for you from a manufactured on demand supplier.
Reviews
From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)
From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)
