Overview

This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Embryo Models In Vitro: Methods and Protocols serves as an ideal guide to these exciting and innovative advances in developmental biology.

Full Product Details

Author:   Magdalena Zernicka-Goetz ,  Kursad Turksen
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2767
Weight:   0.831kg
ISBN:  

9781071636855

ISBN 10:   1071636855
Pages:   323
Publication Date:   07 February 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Generation of Human Blastoids from Naïve Pluripotent Stem Cells             Yulei Wei, Kun Liu, Carlos A. Pinzon-Arteaga, Deirdre Logsdon, Leqian Yu, Ye Yuan, and Jun Wu   2. Human Pre-Gastrulation Embryo Culture in 3D Condition             Lifeng Xiang, Yu Yin, Gaohui Shi, and Tianqing Li   3. Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System             Yu-Xin Luo and Yang Yu   4. Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models             Hao Wu, Yue Wang, and Hongmei Wang   5. Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology             Hao Wu, Yue Wang, and Hongmei Wang   6. Endometrial Assembloids to Model Human Embryo Implantation In Vitro             Thomas M. Rawlings, Maria Tryfonos, Komal Makwana, Deborah M. Taylor, Jan J. Brosens, and Emma S. Lucas   7. Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE)             Yi Zheng   8. Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells             Rowan M. Karvas and Thorold W. Theunissen   9. Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells             Amitesh Panda, Thi Xuan Ai Pham, Sherif Khodeer, and Vincent Pasque   10. Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells             Yuchuan Miao, Margarete Diaz-Cuadros, and Olivier Pourquié   11. Generation of Stem Cell-Based Mouse Embryo-Like Structures             Charlotte E. Handford, Pallavi Panda, Ikbal B. Mohammad Choudhury, Gianluca Amadei, and Magdalena Zernicka-Goetz   12. Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond             Zachary T. Olmsted, Maria Belen Paredes-Espinosa, and Janet L. Paluh   13. Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts             Sharon Arcuri, Georgia Pennarossa, Sergio Ledda, Fulvio Gandolfi, and Tiziana A.L. Brevini   14. Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast             Asha Shaji Antony, Devika AS, and Smita Sudheer   15. Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos             Savana Biondic, Cheng Zhao, Michael Hagemann-Jensen, Stewart J. Russell, Katherine Vandal, Jesica Canizo, Clifford L. Librach, and Sophie Petropoulos   16. Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas             San Kit To, Bradley Balaton, and Vincent Pasque   17. Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL             Tiam Heydari and Peter W. Zandstra   18. ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis             Ceren Pajanoja and Laura Kerosuo   19. Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence             Jesica Canizo, Savana Biondic, Katherine Vandal Lenghan, and Sophie Petropoulos   20. Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics             Katherine Vandal, Savana Biondic, Jesica Canizo, and Sophie Petropoulos   21. Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos             Jesica Canizo, Katherine Vandal, Savana Biondic, and Sophie Petropoulos

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