Overview

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.

Full Product Details

Author:   Biji T. Kurien ,  R. Hal Scofield
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1855
Weight:   1.602kg
ISBN:  

9781493987924

ISBN 10:   1493987925
Pages:   523
Publication Date:   14 November 2018
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface... Table of Contents... Contributing Authors... 1. How It All Began: A Personal History of Gel-Electrophoresis Oliver Smithies 2. Introduction to Protein Electrophoresis Pothur R. Srinivas 3. Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation J.P. Dean Goldring 4. Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis Ewa I. Miskiewicz and Daniel J. MacPhee 5. The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis Thierry Rabilloud 6. Cellulose-Acetate Electrophoresis of Hemoglobin Ramesh Kumar and Wilbert A. Derbigny 7. Native Polyacrylamide gels Claudia Arndt, Stefanie Koristka, Anja Feldmann, and Michael Bachmann 8. Isoelectric Focusing on Non-Denaturing Gels Biji T. Kurien and R. Hal Scofield 9. Determination of protein Molecular weights on SDS-PAGE Hiroyuki Matsumoto, Hisao Haniu, and Naoka Komori 10. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, and Maoka Komori 11. Cationic Electrophoresis Engelbert Buxbaum 12. Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients Bre'Ana Byrd and Huyen Tran 13. SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins Christian Reichel, Gunter Gmeiner, Philipp Reihlen, Mario Thevis, and Wilhelm Schanzer 14. Tricine-SDS-PAGE Syed R. Haider, Helen J. Reid, and Barry L. Sharp 15. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, and Jean M. H. Van den Elsen 16. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies Gyorgy Csako 17. Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-Agarose Marion L. Greaser and Chad M. Warren 18. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis Henghang Tsai and Hon-chiu Eastwood Leung 19. Two-Dimensional Difference Gel Electrophoresis Malachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden 20. Immunoelectrophoresis: A Method with Many Faces Gyorgy Csako 21. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular Mass Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa 22. Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide Bonds Ronald Saraswat and Brian McDonagh 23. Identification of Proteins on Archived 2D Gels Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori 24. Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing Yaser Dorri 25. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols Mustafa Celebier, Clara Ibanez, Carolina Simo, and Alejandro Cifuentes 26. Characterization of New Cyclic D, L - - Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry Maria Damaris Cortez-Diaz, Fanny d'Orlye, and Anne Varenne 27. Microchip Electrophoresis , with Respect to Profiling of Ass Peptides in the Cerebrospinal Fluid of Patients with Alzheier's Disease Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy 28. Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift Karen A. Lewis, Sarah E. Altschuler, and Deborah S. Wuttke 29. Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down Assay Bhupinder Singh and Swapan K. Nath 30. Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes Jennifer A. Ream, L. Kevin Lewis, and Karen A. Lewis 31. Applications of Immobilized Metal affinity Electrophoresis Bao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta 32. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids Gyorgy Csako 33. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann 34. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting Edward P. Trieu and Ira N. Targoff 35. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin 36. Cell Surface Protein Biotinylation for SDS-PAGE Analysis Giuliano Elia 37. Isolation of Proteins from Polyacrylamide Gels Stefanie Koristka, Claudia Arndt, Ralf Bergmann, and Michael Bachmann 38. Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures Robert G.E. Krause and J.P. Dean Goldring 39. Protein Extraction from Gels: A Brief Review Biji T. Kurien, Rachna Aggarwal, and R. Hal Scofield 40. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome Xianchun Wang and Songping Liang 41. Ultra-Rapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis Rohit Thomas and Biji T. Kurien 42. A Brief Review of Other Notable Electrophoretic Methods James D. Fesmire 43. Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit Kristi A. Koelsch 44. Artifacts and Common Errors in Protein Gel Electrophoresis Biji T. Kurien and R. Hal Scofield

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