Overview

This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.

Full Product Details

Author:   John Kuo
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed.
Volume:   v. 369
Dimensions:   Width: 15.20cm , Height: 3.30cm , Length: 22.90cm
Weight:   1.279kg
ISBN:  

9781588295736

ISBN 10:   1588295737
Pages:   624
Publication Date:   27 February 2007
Audience:   College/higher education ,  Undergraduate
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Awaiting stock  

Table of Contents

CONTENTS Electron Microscopy: Methods and Protocols (2nd Edition) METHODS IN MOLECULAR BIOLOGY Volume 117 1. Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells John J. Bozzola (USA) 2. Processing Biological Tissues for Ultrastructural Study Jose A. Mascorro (USA) and John J. Bozzola (USA) 3. Processing Plant Tissues for Ultrastructural Study John Kuo and Ivan Kuo (Australia) 4. Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy Paul Webster (USA) 5. Ultramicrotomy: for Biological Electron Microscopy Herbert K. Hagler (USA) 6. Post Staining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols E. Ann Ellis (USA) 7. Negative Staining of Thinly Spread Biological Samples J. Robin Harris (Germany) 8. Recent Advances in High Pressure Freezing: Equipment and Specimen Loading Methods Kent L McDonald, Mary Morphew, Paul Verkade, and Thomas Muller-Reichert (USA/Germany) 9. Cryoultramicrotomy: Cryo-Electron Microscopy of Vitreous Sections Dimitri Vanhecke, Luca Studer and Daniel Studer (Switzerland) 10. Cell-free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy Margaret Coughlin, William M. Brieher and Ryoma Ohi (USA) 11. Electron Microscopy In Situ Hybridization - Tracking DNA and RNA Sequences at High Resolution Du_an Cmarko and Karel Koberna (Chezvakea) 12. Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections Heinz Schwarz and Bruno M. Humbel (Germany/Netherlands) 13. Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry Paul Webster and Alexandre Webster (USA) 14. F-TEM mages: Integrating Fluorescence Microscopy with Transmission Electron Microscopy Paul A. Sims and Jeff D. Hardin (USA) 15. Quantitative Immunoelectron Microscopy: Alternative Ways of Assessing Subcellular Patterns of Gold Labelling Terry M. Mayhew (England) 16. Electron Crystallography of Membrane Proteins Hui-Ting Chou, James Evans and Henning Stahlberg (USA) 17. Cryo-Electron Microscopy of Icosahedral Virus Particle Wen Jiang and Wah Chiu (USA) 18. 3D Reconstructions of Chromosomes with Electron Tomography Peter Engelhardt (Finland) 19. Whole-Mount Immuno-Electron Tomography of Chromosomes and Cells Peter Engelhardt, Jari Merilainen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui and Veli-Pekka Lehto (Finland/Japan) 20. 3-D Cryo-Transmission Electron Microscopy of Cells and Organelles Michael Marko and Chyong-Ere Hsieh (USA) 21. Electron Energy-Loss Spectroscopy (EELS) as a Tool for Elemental Analysis in Biological Specimens Nadine Kapp, Peter Gehr, Daniel Studer and Marianne Geiser (Switzerland) 22. Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens John J. Bozzola (USA) 23.Variable Pressure (VPSEM) and Environmental Scanning Electron Microscopy (ESEM): Imaging of Biological Samples Brendan J. Griffin (Australia) 24. Cryo-planing Technique for Visualizing the Distribution of Water in Woody Tissue by Cryo-Scanning Electron Microscopy Yasuhiro Utsumi and Yuzou Sano (Japan) 25. X-ray Microanalysis in the Scanning Electron Microscope Godfrield M Roomans and Anca Dragomir (Sweden) 26. Botanical SEM X-ray Microanalysis in Cryo-Scanning Electron Microscopy Beat Frey (Switzerland) 27. Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research Nicholas P. Lockyer (England) 28. Using SIMS and MIMS in Biological Materials: Application to Higher Plants Nicole Grignon (France)

Reviews

This is an excellent book, with well-written chapters. The protocols are easy to follow and well organized. This book is highly recommended for beginners to advanced level microscopists. -Micron

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