Overview
This readily reproducible collection of cutting-edge methods for the study of eicosanoids concentrates on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
Full Product Details
Author: Elias A Lianos
Publisher: Humana Press Inc.
Imprint: Humana Press Inc.
Edition: 1999 ed.
Volume: 120
Dimensions:
Width: 15.20cm
, Height: 1.70cm
, Length: 22.90cm
Weight: 1.020kg
ISBN: 9780896036673
ISBN 10: 0896036677
Pages: 296
Publication Date: 25 March 1999
Audience:
College/higher education
,
Professional and scholarly
,
Undergraduate
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: In Print 
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Table of Contents
Use of a Retroviral Vector for Expression of a Foreign Gene, Brent W. Miller and Aubrey R. Morrison. Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues, William S. Powell. Assessment of Cyclooxygenase RNA Expression by Northern Hybridization, Zhonghong Guan and Aubrey R. Morrison. Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin, ShaAvhree Y. Buckman, Alane T. Koki, Dorothy A. Edwards, and Alice P. Pentland. Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography, Mukut Sharma, Beatrice Hucke, and Elias A. Lianos. Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry, M. Kerry O'Banion and John A. Olschowka. Assessment of Cyclooxygenase Protein Expression by Western Blotting, Zhonghong Guan and Aubrey R. Morrison. Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells, Alfred N. Fonteh. Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction, Alfred N. Fonteh, William Reed, and James M. Samet. Enzyme Immunoassays (EIA) of Eicosanoids, Kingsley Yin and Lloyd J. Forman. Transcellular Regulation of Eicosanoid Biosynthesis, Karsten Gronert, Clary B. Clish, Mario Romano, and Charles N. Serhan. Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry,Samuel C. Blackman, Catherine Borg, David C. Yeomans, and Guy C. Le Breton. Epoxyeicosatrienoic Acids: Biosynthesis, Regulation, and Actions, John D. Imig. Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts,Srinivasa T. Reddy and Harvey R. Herschman. Assessment of 5-Lipoxygenase Activity and Cellular Distribution, Robert A. Lepley. Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids, Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong. Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition, Eric G. Spokas, Nancy S. Nicholson, Osman D. Suleymanov, Gerald M. Walsh, and David Tuffin. Novel Eicosanoids: Isoprostanes and Related Compounds, L. Jackson Roberts II, Cynthia J. Brame, Yan Chen, and Jackson D. Morrow. Index.
Reviews
...well-produced, timely book... -The Quarterly Review of Biology A very useful book for all specialists on eicosanoid acids, which was lacking until now in the literature. - Cellular and Molecular Biology
well-produced, timely book - The Quarterly Review of Biology A very useful book for all specialists on eicosanoid acids, which was lacking until now in the literature. - Cellular and Molecular Biology
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