Overview

A multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx). Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology - from the purification of the toxin to the effects of Stx on various host cell functions - and for exploring STEC-mediated disease in various animal models.

Full Product Details

Author:   Dana Philpott ,  Frank Ebel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2003 ed.
Volume:   73
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.686kg
ISBN:  

9780896039391

ISBN 10:   0896039390
Pages:   352
Publication Date:   26 September 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections.- Methods for Detection of STEC in Humans.- Serological Methods for the Detection of STEC Infections.- Detection and Characterization of STEC in Stool Samples Using PCR.- Molecular Typing Methods for STEC.- STEC in the Food Chain.- STEC as a Veterinary Problem.- Cellular Microbiology of STEC Infections.- Analysis of Pathogenicity Islands of STEC.- Generation of Isogenic Deletion Mutants of STEC.- Generation of Monoclonal Antibodies Against Secreted Proteins of STEC.- Microscopic Methods to Study STEC.- Detection and Characterization of EHEC-Hemolysin.- Shiga Toxin Receptor Glycolipid Binding.- Methods for the Purification of Shiga Toxin 1.- Methods for the Identification of Host Receptors for Shiga Toxin.- Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport.- Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin.- Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways.- Interaction of Shiga Toxin with Endothelial Cells.- Shiga Toxin Interactions with the Intestinal Epithelium.- Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes.- Animal Models for STEC-Mediated Disease.- Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC.- Bovine Escherichia coli O157:H7 Infection Model.

Reviews

The authors should be thanked for providing this valuable resource tool that is highly recommended to microbiologists, molecular biologists and to all who are interested in bacterial toxin action. All medical libraries should house a copy of this text. The book also will serve as an excellent teaching guide for students and fellows particularly in postgraduate programs. - Folia Microbiologica

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