Overview

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Full Product Details

Author:   Michelle S. Giedt ,  Tina L. Tootle
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2626
Weight:   1.063kg
ISBN:  

9781071629697

ISBN 10:   1071629697
Pages:   447
Publication Date:   31 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. The Vast Utility of Drosophila Oogenesis             Michelle S. Giedt and Tina L. Tootle   2. Visualization and Quantification of Drosophila Larval Ovaries             Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Alejandro Campoy-Lopez, and Acaimo González-Reyes   3. Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries             Julie A. Merkle   4. Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary             Daniel N. Phipps, Amanda M. Powell, and Elizabeth T. Ables   5. Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication             Lesley N. Weaver   6. Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes             Tingting Duan, Felipe Rodriguez-Tirado, and Pamela K. Geyer   7. Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells             Anna E. Williams and Elizabeth T. Ables   8. Tracking Follicle Cell Development             Adrianna Soriano, Christopher Petit, Savannah Ryan, and Jennifer C. Jemc   9. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells             Mitchell T. Anderson, Kristin Sherrard, and Sally Horne-Badovinac   10. Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration             Yujun Chen, Nirupama Kotian, and Jocelyn A. McDonald   11. Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis             Jonathan A. Jackson, Jasmin Imran Alsous, and Adam C. Martin   12. Visualizing Lipid Droplets in Drosophila Oogenesis             Roger P. White and Michael A. Welte   13. Assessing Ovulation in Drosophila melanogaster             Andrew Beard, Rebecca Oramas, and Jianjun Sun   14. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments             Allison L. Zajac, Audrey M. Williams, and Sally Horne-Badovinac   15. Detection and Assessment of Wolbachia pipientis Infection             Lindsay B.M. Nevalainen and Irene L.G. Newton   16. Measuring Transposable Element Activity in Adult Drosophila Ovaries             Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Csilla Abonyi, and Péter Vilmos   17. Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing             Nathaniel Meyer, Jobelle Peralta, and Todd Nystul   18. Chromatin Immunoprecipitation Experiments from Drosophila Ovaries             Maria Sokolova and Maria K. Vartiainen   19. Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary             Ildikó Kristó, Péter Borkúti, Zoltán Kovács, Anikó Szabó, Szilárd Szikora, and Péter Vilmos   20. STAMP: Spatio-Temporal Association Mapping of Proteins             Yuanbing Zhang, Bo Zhang, and Ji-Long Liu   21. Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research             Jayson A. Cortes and Christina I. Swanson   22. Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research             Dara M. Ruiz-Whalen, Christopher P. Aichele, Ebony R. Dyson, Katherine C. Gallen, Jennifer V. Stark, Jasmine A. Saunders, Jacqueline C. Simonet, Erin M. Ventresca, Isabela M. Fuentes, Nyellis Marmol, Emly Moise, Benjamin C. Neubert, Devon J. Riggs, Ava M. Self, Jennifer I. Alexander, Ernest Boamah, Amanda J. Browne, Iliana Correa, Maya J. Foster, Nicole Harrington, Troy J. Holiday, Ryan A. Henry, Eric H. Lee, Sheila M. Longo, Laurel D. Lorenz, Esteban Martinez, Anna Nikonova, Maria Radu, Shannon C. Smith, Lindsay A. Steele, Todd I. Strochlic, Nicholas F. Archer, Y. James Aykit, Adam J. Bolotsky, Megan Boyle, Jennifer Criollo, Oren Eldor, Gabriela Cruz, Valerie N. Fortuona, Shreeya D. Gounder, Nyim Greenwood, Kayla W. Ji, Aminah Johnson, Sophie Lara, Brianna Montanez, Maxwell Saurman, Tanu Singh, Daniel R. Smith, Catherine A. Stapf, Tarang Tondapu, Christina Tsiobikas, Raymond Habas, and Alana M. O’Reilly

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