Overview

The fruit fly drosophila melanogaster serves as the classic model for the demonstration of the basic phenomena of genetics. With its easily recognizable genetic markers it is also ideally suited as a test organism for screening newly developed substances for toxicity and mutagenicity. The practical course manual on drosophila includes all experiments on formal genetics which can be carried out without expensive equipment. The experiments are described in enough details that teachers and students with little previous experience in drosophila techniques can complete them successfully. A results and answers section helps students to control their experiments and guides them through scientific reasoning. Routine laboratory procedures can be established following this manual and it serves as a prerequisite to perform more sophisticated experiments including techniques of molecular genetics.

Full Product Details

Author:   Ulrich Graf ,  Nancy van Schaik ,  Friedrich E. Würgler ,  F.E. Wuergler (University of Zurich, Switzerland)
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Dimensions:   Width: 15.50cm , Height: 1.30cm , Length: 23.50cm
Weight:   0.388kg
ISBN:  

9783540543275

ISBN 10:   3540543279
Pages:   239
Publication Date:   16 March 1992
Audience:   College/higher education ,  Undergraduate
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

1. General.- 1.1 Why Drosophila?.- 1.2 Basic Equipment.- 1.3 Genetic Terminology.- 1.4 The Biology of Drosophila melanoqaster.- 1.5 Methods for Culturing and Studying Drosophila melanoqaster.- 1.6 List of Genetic Symbols and Stocklist.- 1.7 General Books on Drosophila.- 2. Morphology of Drosophila Melanogaster.- 2.1 Eggs and Embryos.- 2.2 L3 Larvae.- 2.3 Pupae.- 2.4 Adult Flies.- 2.5 Some Mutants.- 3. Transmission Genetics.- 3.1 Dihybrid Cross with Independent Assortment.- 3.2 Sex-Linked Inheritance.- 3.3 Dihybrid Test Cross with Linked Genes.- 3.4 Sex Determination.- 3.5 Genetic Localization of Mutations Within the Genome.- 3.6 Mapping on a Chromosome.- 3.7 Segregation of Compound Chromosomes.- 3.8 Meiotic Mutants.- 4. Phenogenetics.- 4.1 Temperature Effect on Expression of Phenotype.- 4.2 Temperature Effect on a Homeotic Mutant.- 4.3 Mutants with Abnormal Eye Color.- 4.4 Chromatographic Analysis of Eye Color Mutants.- 4.5 Transplantation of Eye Imaginai Discs.- 4.6 Supplementation of an Eye Color Mutant.- 4.7 Genetic Complementation and Allelism.- 4.8 Transposable Elements.- 5. Mutation Genetics.- 5.1 Induction and Detection of Sex-Linked Recessive Lethals.- 5.2 Induction and Detection of Reciprocal Translocations.- 5.3 Mutagen-Induced Sex Chromosome Losses.- 5.4 Somatic Mutation and Mitotic Recombination.- 6. Population Genetics.- 6.1 Determination of Allele Frequencies in Populations in Hardy-Weinberg Equilibrium.- 6.2 Variations in Populations with Natural and Artificial Selection.- 6.3 Mating Behavior.- 6.4 Mating Preferences.- 7. Cytology and Cytogenetics.- 7.1 Microscopic Analysis of Mitotic and Polytene Chromosomes.- 7.2 Balancer Chromosomes.- 8. Molecular Biology of Drosophila: An Overview.- 9. Results and Answers.- 10. Subject Index.

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