Overview

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision.

Full Product Details

Author:   M. Maluszynski ,  Kenneth Kasha ,  B.P. Forster ,  I. Szarejko
Publisher:   Springer
Imprint:   Springer
Edition:   Softcover reprint of the original 1st ed. 2003
Weight:   0.730kg
ISBN:  

9789048163939

ISBN 10:   9048163935
Pages:   428
Publication Date:   12 December 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

1. Production of doubled haploids in crop plants. An introduction.- 2.1. Doubled haploid production in barley using the Hordeum bulbosum (L.) technique.- 2.2. The Hordeum bulbosum (L.) method.- 2.3 Anther culture in barley.- 2.4. Barley anther culture.- 2.5. Anther culture for doubled haploid production in barley (Hordeum vulgare L.).- 2.6. Barley isolated microspore culture protocol.- 2.7. Barley isolated microspore culture (IMC) method.- 2.8. Doubled haploid production in wheat through wide hybridization.- 2.9. Protocol of wheat (Triticum eastivum L.) anther culture.- 2.10. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.).- 2.11. Wheat anther culture.- 2.12. Haploid wheat isolated microspore culture protocol.- 2.13. Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals.- 2.14. Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis.- 2.15. Anther culture of maize (Zea mays L.).- 2.16. Laboratory protocol for anther culture technique in rice.- 2.17. Triticale anther culture.- 2.18. Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm.).- 2.19. Protocol of triticale (x Triticosecale Wittmack) microspore culture.- 2.20. Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize.- 2.21. Protocol for rye anther culture.- 2.22. Microspore culture of rye.- 2.23. Oat haploids from wide hybridization.- 2.24. Haploid and doubled haploid production in dumm wheat by wide hybridization.- 2.25. Haploid and doubled haploid production in dumm wheat by anther culture.- 2.26. Anther culture and isolated microspore culture in timothy.- 2.27. Doubled haploid induction in ryegrass and other grasses.- 2.28. Microspore culture in rapeseed (Brassica napus L.).- 2.29. Protocol for broccoli microspore culture.- 2.30. Microspore culture of Brassica species.- 2.31. Protocol for microspore culture in Brassica.- 2.32. Anther and microspore culture in tobacco.- 2.33. Haploid production of potatoes by anther culture.- 2.34. Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids.- 2.35. Potato haploid technologies.- 2.36. Anther culture of linseed (Linum usitatissimum L.).- 2.37. Doubled haploid production of sugar beet (Beta vulgaris L.).- 2.38. Asparagus microspore and anther culture.- 2.39. In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.).- 2.40. Haploid induction in onion via gynogenesis.- 2.41. In vitro androgenesis in apple.- 2.42. Doubled haploid production in poplar.- 2.43. Oak anther culture.- 2.44. Haploids and doubled haploids in Citrus spp..- 3. Published protocols for other crop plant species.- 4.1. Doubled haploids in breeding.- 4.2. Doubled haploid mutant production.- 4.3. Barley microspore transformation protocol by biolistic gun.- 4.4. Doubled haploids in genetic mapping and genomics.- 5.1. Cytogenetic tests for ploidy level analyses — chromosome counting.- 5.2. Ploidy determination using flow cytometry.- 6.1. Major media composition.- 6.2. Basic equipment for maize microspore culture laboratory.- List of contributors.

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