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Author:   Xiaofei Cheng ,  Guanwei Wu
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Volume:   2771
ISBN:  

9781071637043

ISBN 10:   1071637045
Pages:   142
Publication Date:   02 April 2025
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation.- Detection of dsRNA by Acridine Orange Staining.- Isolation of dsRNA from Plants by Cellulose Chromatography.- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column.- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay.- Detection of dsRNA with the Fluorescence In Situ Hybridization (FISH) Assay.- Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana.- Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription.- Inducible Expression of dsRNA in Escherichia coli.- In Vivo Production of dsRNA Using Bacteriophage ϕ6 in Pseudomonas syringae Cit7 Cells.- Bacteria-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management.- Transiently Induce RNA Silencing in Plants Using a TobaccoNecrosis Virus A (TNV-A)-Based dsRNA Production System.- Co-Immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana.- Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana.- Identification of Mycoviruses by dsRNA Extraction.- Production of Double-Stranded RNA In Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing.- Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea.- Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus.

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