DNA Viruses: Methods and Protocols

Author:   Paul M. Lieberman
Publisher:   Humana Press Inc.
Edition:   2005 ed.
Volume:   292
ISBN:  

9781627038102


Pages:   498
Publication Date:   22 November 2014
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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DNA Viruses: Methods and Protocols


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Author:   Paul M. Lieberman
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2005 ed.
Volume:   292
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.783kg
ISBN:  

9781627038102


ISBN 10:   1627038108
Pages:   498
Publication Date:   22 November 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Viral Detection.- Viral Detection.- Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1.- Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR.- Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo.- Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo.- Reporter Cell Lines for the Detection of Herpes Simplex Viruses.- Virus Structure And Imaging.- Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy.- Studying the Structure of Large Viruses With Multiresolution Imaging.- Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence.- Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy.- FTIR Microscopy Detection of Cells Infected With Viruses.- Virus Entry.- The JC Virus-Like Particle Overlay Assay.- Analysis of Fusion Using a Virus-Free Cell Fusion Assay.- Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells.- Gene Expression.- Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence.- Identification and Characterization of Herpesviral Immediate-Early Genes.- Replication And Genome Maintenance.- Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids.- DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins.- Pathogenesis.- Pre-B-Cell Colony Formation Assay.- Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis.-Complex Cell Systems.- Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection.- Human Papillomavirus Type 31 Life Cycle Methods for Study Using Tissue Culture Models.- Recombinant Genetics.- Molecular Genetics of Herpesviruses A Recombinant Technology Approach.- Molecular Genetics of DNA Viruses Recombinant Virus Technology.- Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis.- Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology.- Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs.- Computation/Systems Biology Of Viruses.- Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray.- Real-Time Quantitative PCR Analysis of Viral Transcription.- Rapid Screening of Chemical Inhibitors That Block Processive DNA Synthesis of Herpesviruses.

Reviews

Good presentation...laboratories working with DNA viruses will find ample useful material... - Doody's Health Sciences Book Review Journal


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