Overview

DNA Sequencing Protocols, Second Edition, empowers researchers with the tools and knowledge required to become a sequencing expert. It covers a wide range of DNA sequencing technologies in common usage in molecular biology laboratories. It acts as a laboratory guide to DNA sequencing and it helps students and researchers to understand the instrumentation (ABI, ALF & LiCor) and chemistries involved. The reader is also given internet addresses for Genome sequencing sites and step by step guides to the use of sequence analysis software and databases i. e. for BLAST and NIX searches. The book also reviews some of the instrumentation available for automation of DNA sequencing processes which is in more widespread use in many research groups. Some of the protocols covered in detail include, M13 cloning and sequencing; Primer design; PCR product sequencing; Cycle sequencing; Solid phase sequencing; Shotgun sequencing; Fluorescent sequencing for 310, 373, 377, ALF, LiCor; Heterozygote mutation detection; Automation, and; Sequence databases and the internet. The book will also be useful to clinicians with research interests to update them on current DNA sequencing methods, applications and future prospects. DNA sequencing has become an essential tool for molecular diagnostics with the completion of many genome project.

Full Product Details

Author:   Colin A Graham ,  Alison J.M. Hill
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2001
Volume:   167
Dimensions:   Width: 15.20cm , Height: 2.20cm , Length: 22.90cm
Weight:   0.421kg
ISBN:  

9780896037212

ISBN 10:   0896037215
Pages:   256
Publication Date:   10 January 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Introduction to DNA Sequencing. The Universal Primers and the Shotgun DNA Sequencing Method. M13 Sequencing. Primer Design and Primer-Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene. Shotgun DNA Sequencing. Cycle Sequencing. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. Fluorescent Sequencing Protocols for the ALF. Fluorescent Sequencing for Heterozygote Mutation Detection. Sequence Databases and the Internet. DNA Sequencing by Capillary Array Electrophoresis. Index.

Reviews

From Reviews of the First Edition continues the standard of excellent laboratory manuals that has been set by earlier volumes Many of the protocols are significantly easier to follow than comparable protocols in other lab handbooks provides one of the best collections of manuals for DNA sequencing applications - FEBS Letters a valuable addition to the library of any laboratory that is actively engaged in DNA sequencing - Quarterly Review of Biology any laboratory undertaking a large DNA sequencing project would benefit from having this manual - Trends in Biotechnology

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