Overview

Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.

Full Product Details

Author:   Sonya Vengrova ,  Jacob Dalgaard
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2015
Volume:   1300
Dimensions:   Width: 17.80cm , Height: 1.90cm , Length: 25.40cm
Weight:   7.938kg
ISBN:  

9781493925957

ISBN 10:   1493925954
Pages:   295
Publication Date:   28 April 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells.- Microscopy Techniques to Examine DNA Replication in Fission Yeast.- High-Resolution Analysis of Mammalian DNA Replication Units.- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing.- Measuring DNA Content by Flow Cytometry in Fission Yeast.- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast.- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection.- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo.- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae.- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin.- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors.- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism.- Single-Molecule Observation Of Prokaryotic DNA Replication.- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis.- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions