Overview

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies. 

Full Product Details

Author:   Marcos Simoes-Costa
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2599
Weight:   0.758kg
ISBN:  

9781071628461

ISBN 10:   1071628461
Pages:   284
Publication Date:   26 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. A DNA pull-down assay with diversity forms of competitor for detecting or evaluating protein-DNA interactions Hongyan Sui and Tomozumi Imamichi   2. Enhanced yeast one-hybrid assays to study protein-DNA interactions Anna Berenson, Juan Ignacio, and Fuxman Bass   3. Chromatin Immunoprecipitation on Fixed Tissues and Cell Lines Dimitrius Pramio and Deborah Schechtman   4. The ChIP-exo method to identify genomic locations of DNA-binding Proteins at Near Single Base-Pair Resolution Ssu-Yu Yeh and Ho Sung Rhee   5. ChIP-seq Protocol for invVitro Cell Differentiation Systems Jacqueline Copeland, Fjodor Merkuri, and Marcos Simoes-Costa   6. Profiling Accessible Chromatin and Nucleosomes in the Mammalian Genome Hee Woong Lim and Makiko Iwafuchi   7. Dissecting Locus-Specific Chromatin Interactions by CRISPR Capture Giovanni A. Botten, Michael Lee Jr., and Jian Xu   8. CUT&RUN to Profile Chromatin-Bound Proteins in Primary Mouse Neural Progenitor Cells Yurika Matsui and Jamy C. Peng   9. Easy Hi-C: A Low Input Method for Capturing Genome Organization Leina Lu and Fulai Jin   10. Single Molecule Imaging of DNA Protein Interactions Using DNA Curtains J. Brooks Crickard   11. Quantifying the Binding and Target-Search Kinetics of Transcriptional Regulatory Factors by Live-Cell Single-Molecule Tracking Steven Ingersoll, Kyle Brown, Brian Ma, and Xiaojun Ren   12. Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles Emeleeta A. Paintsil and Emma A. Morrison   13. Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA Erin F. Hammonds and Emma A. Morrison   14. Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering Karmella A. Haynes and J. Harrison Priode 15. Mapping Transcription Regulation with Run-on and Sequencing Data Using The Web-Based tfTarget Gateway Nating Wang, Zhong Wang, Charles G. Danko, and Tinyi Chu   16. In embryo Gene Reporter Assays for Evaluation of Cis-Regulatory Regions Carolina Purcell Goes, Tatiane Y. Kanno, and C.Y. Irene Yan   17. In situ Hybridization as a Method to Examine Gene Regulatory Activity in vivo Rhea R Datta and Pinar Onal   18. In situ Hybridization as a Method to Examine Gene Regulatory Activity in vivo Rhea R Datta and Pinar Onal   19. Fishing for developmental regulatory regions: Zebrafish tissue-specific ATAC-seq Rekha M. Dhillon-Richardson, Alexandra K. Haugan, and Megan L. Martik  

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions