Overview
DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. Such biochips enable the use of advanced fabrication, detection, and data mining technologies that allow data gathering at an unprecedented rate. The first chapter is an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips. The next two chapters describe the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. Chapter 4 describes assay optimization for enzymatic assays and chapter 5 antisense scanning arrays. This is followed by a chapter on the manufacture of molecular arrays using ink-jet printing technology. Chapter 7 describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics. Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology in pharmacogenomics. This Practical Approach book is a comprehensive overview of the new and expanding field of DNA microarray technology and will be invaluable to any researcher interested in the use of biochips.
Full Product Details
Author: Mark Schena (Department of Biochemistry, Department of Biochemistry, Stanford University, USA)
Publisher: Oxford University Press
Imprint: Oxford University Press
Volume: 205
Dimensions:
Width: 15.70cm
, Height: 1.40cm
, Length: 23.50cm
Weight: 0.409kg
ISBN: 9780199637768
ISBN 10: 0199637768
Pages: 232
Publication Date: 24 June 1999
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: To order 
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.
Table of Contents
1: Mark Schena and Ronald W. Davis: Genes, genomes, and chips: Introduction; Information content of genomes; microarray analysis; Applications of microarrays; Chips and pharmacogenomics; Summary; References 2: Mack J. Schermer: Confocal scanning microscopy in microarray detection: Introduction - microarrays, fluorescence, and detection; Sample handling; Confocal scanning of microarrays; Wavelength discrimination - minimizing the background image; Detectors; Signal processing and instrument control; Instrument performance measures; Scanarray (registered trademark) confoccal microarray scanners; References 3: Stanley F. Nelson and Christopher T. Denny: Representational differences analysis and microarray hybridization for efficient cloning and screening of differentially expressed genes: Introduction; RDA; Generation of libraries from RDA subtractions; Microarray fabrication; microarray hybridization; Differential expression screening with microarrays; Summary; References 4: Stephen Case-Green, Clare Pritchard, and Edwin M. Southern: Use of oligonucleotide arrays in enzymatic assays: assay optimization: Introduction; Fabrication of arrays; Preparation of target DNA; Hybridization to arrays; Enzyme catalysed extension reactions; Variations on protocols; References 5: John K. Elder, Martin Johnson, Natalie Milner, Kalim U. Mir, Muhammad Sohail, and Edwin M. Southern: Antisense oligonucleotide scanning arrays: Introduction; Array fabrication; Acknowledgements; References 6: Thomas P. Theriault, Scott C, Winder, and Ronald C, Gamble: Application of ink-jet printing technology to the manufacture of molecular arrays: Introduction; Ink-jet technology; Molecular array fabrication by synthesis; Molecular array fabrication by deposition; Conclusion; Acknowledgements; References 7: Ranelle C. Salunga, Hongqing Guo, Lin Luo, Anton Bittner, K. C. Joy, Jim R. Chambers, Jackson S. Wan, Michael R. Jackson, and Mark G. Erlander: Gene expression analysis via cDNA microarrays of laser capture microdissected cells from fixed tissue: Ranelle C. Salunga, Hongqing Guo, Lin Luo, Anton Bittner, K. C. Joy, Jim R. Chambers, Jackson S. Wan, Michael R. Jackson, and Mark G. Erlander: Introduction; Laser capture microdissection; Generation of aRNA - first round; cDNA microarrays; Acknowledgements; References 8: Joel Lloyd Bellenson: Expression data and the bioinformatics challenges: Genes/proteins; The raw data pipeline; The reference data warehouse; The structure of molecular information; Medial lexicon and donor sample registration; Data mining an expression data mart; Conclusions; References 9: Michael J. Heller, Eugene Tu, Anita Holmsen, Ronald G. Sosnowski, and James O'Connell: Active microelectronic arrays for DNA hybridization analysis: Introduction; Background on electronic array and hybridization technology; Active microelectronics array fabrication; Point mutation analysis on active electronic arrays; Microfabrication of active DNA chips; Example of single base discrimination analysis; Conclusions; References 10: Renu A. Heller, John Allard, Fengrong Zuo, Christopher Lock, Stacy Wilson, Paul Klonowski, Hans Gmuender, Harold Van Wart, and Robert Booth: Gene chips and microarrays: applications in disease profiles, drug target discovery, drug action and toxicity: Introduction; Methods; Results; Discussion; Acknowledgements; References Appendix Index
Reviews
DNA Microarrays (biochips) are small glass chips embedded with ordered rows of DNA which allow data to be gathered quickly. This volume provides a guide to this new technology, addressing the use of confocal scanning in microarray detection, techniques for cloning and screening of differentially expressed genes, the optimization of enzymatic assays using oligonucleotide arrays, antisense oligonucleotide scanning arrays, the production of molecular arrays using ink-jet printing technology, gene expression analysis via cDNA microarrays, the use of expression data in bioinformatics, active microelectronic arrays for DNA hybridization analysis, and the applications of microarray technology in pharmacogenomics. In each article, methodological details and current applications are described. Contributors primarily consist of biochemists and researchers in pharmaceutical companies in the US, Switzerland, and the UK. --SciTech Book News<br>
DNA Microarrays (biochips) are small glass chips embedded with ordered rows of DNA which allow data to be gathered quickly. This volume provides a guide to this new technology, addressing the use of confocal scanning in microarray detection, techniques for cloning and screening of differentially expressed genes, the optimization of enzymatic assays using oligonucleotide arrays, antisense oligonucleotide scanning arrays, the production of molecular arrays using ink-jet printing technology, gene expression analysis via cDNA microarrays, the use of expression data in bioinformatics, active microelectronic arrays for DNA hybridization analysis, and the applications of microarray technology in pharmacogenomics. In each article, methodological details and current applications are described. Contributors primarily consist of biochemists and researchers in pharmaceutical companies in the US, Switzerland, and the UK. --SciTech Book News
'...it's an essential purchase and probably the cheapest one a microarraying lab will make' Microbiology Today '...With its well-laid out protocols, the book manages to instruct the reader well and should be accessible to both novice and expert molecular biologists alike' Aslib Book Guide
'...With its well-laid out protocols, the book manages to instruct the reader well and should be accessible to both novice and expert molecular biologists alike' Aslib Book Guide '...it's an essential purchase and probably the cheapest one a microarraying lab will make' Microbiology Today
Author Information
Tab Content 6
Author Website:
Customer Reviews
Recent Reviews
No review item found!
Add your own review!
Countries Available
All regions
|
