Overview

DNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.

Full Product Details

Author:   Ken Mills ,  BH Ramsahoye (Humana author USA)
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   v. 200
Dimensions:   Width: 15.20cm , Height: 1.80cm , Length: 22.90cm
Weight:   0.446kg
ISBN:  

9780896036185

ISBN 10:   0896036189
Pages:   176
Publication Date:   01 March 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Overview. Nearest-Neighbor Analysis. Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography. Methylation Analysis by Chemical DNA Sequencing. Methylation-Sensitive Restriction Fingerprinting. Restriction Landmark Genome Scanning. Combined Bisulfite Restriction Analysis (COBRA). Differential Methylation Hybridization Using CpG Island Arrays. Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences. Isolation of CpG Islands Using a Methyl-CpG Binding Column. Purification of MeCP2-Containing Deacetylase from Xenopus laevis. DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method. Measuring DNA Demethylase Activity In Vitro. Extracting DNA Demethylase Activity from Mammalian Cells. Index.

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