Overview

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.

Full Product Details

Author:   Garry Scarlett
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2633
Weight:   0.693kg
ISBN:  

9781071630037

ISBN 10:   1071630032
Pages:   249
Publication Date:   01 March 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Classical Recombinant DNA Cloning Ana Mikić, Arqam Alomari, and Darren M. Gowers   2. A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector Robert A. Holland   3. Molecular cloning using in vivo DNA assembly Sandra Arroyo-Urea, Jake F. Watson and Javier García-Nafría   4. Assembling multiple fragments: The Gibson Assembly Luisana Avilan   5. TA cloning approaches to cloning DNA with damaged ends DNA Charlotte Ayling   6. PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis Timothy J. Ragan and Helen A. Vincent   7. Random Mutagenesis by PCR F.A Myers   8. In vitro Site Directed Mutagenesis Michael J. McClellan   9. Xenopus transgenesis using the pGateway system Liliya Nazlamova   10. CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog Anita Abu-Daya and Annie Godwin   11. A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation Sian Angela Martin   12. A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets Robert Gowland and Darren M. Gowers   13. Generation of functional-RNA arrays by in vitro transcription and in situ RNA capture for the detection of RNA-RNA interactions Helen A. Vincent, Charlotte A. Henderson, Daniela Lopes Cardoso and Anastasia J. Callaghan   14. Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry John Brazier   15. Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products George D. Zouganelis and Nikolaos Tairis   16. Next generation Sequencing for mixed samples Angela Beckett and Sam Robson   17. Ethics, Legality and Safety for Geneticists Dr Simon Kolstoe

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