DNA Cloning 4: A Practical Approach: Mammalian Systems
Author:   D. M. Glover (Department of Anatomy and Physiology, University of Dundee) ,  B. D. Hames (Department of Biochemistry and Molecular Biology, University of Leeds) ,  B.D. Hames
Publisher:   Oxford University Press
Edition:   Revised edition
Volume:   164
ISBN:  

9780199634804


Pages:   340
Publication Date:   25 January 1996
Format:   Paperback
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

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DNA Cloning 4: A Practical Approach: Mammalian Systems


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Overview

DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments.This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.

Full Product Details

Author:   D. M. Glover (Department of Anatomy and Physiology, University of Dundee) ,  B. D. Hames (Department of Biochemistry and Molecular Biology, University of Leeds) ,  B.D. Hames
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   Revised edition
Volume:   164
Dimensions:   Width: 15.70cm , Height: 1.70cm , Length: 23.40cm
Weight:   0.514kg
ISBN:  

9780199634804


ISBN 10:   0199634807
Pages:   340
Publication Date:   25 January 1996
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1: High efficiency gene transfer into mammalian cells 2: Construction and characterization of Vaccinia virus recombinants 3: Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells 4: Retroviral vectors 5: Genetic manipulation of embryonic stem cells 6: Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs 7: Genomic and expression analysis of transgenic animals 8: Expression using a defective Herpes simplex virus (HSV-1) vector system 9: Adenovirus vectors

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