Overview

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Full Product Details

Author:   Akash Gautam
Publisher:   Springer Nature Switzerland AG
Imprint:   Springer Nature Switzerland AG
Edition:   1st ed. 2022
Weight:   0.343kg
ISBN:  

9783030942328

ISBN 10:   3030942325
Pages:   203
Publication Date:   31 March 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1-Basic idea about nucleic acids for non-experts   2-General steps during isolation of DNA and RNA   3-Phenol-chloroform DNA isolation method   4-RNA isolation by the guanidinium-acid-phenol method   5-Spin column-based isolation of nucleic acid   6-Isolation of plasmid DNA by alkaline lysis   7-DNA Isolation by Hydrophilic Ionic Liquid Treatment   8-Lithium Chloride based isolation of DNA and RNA   9-Isolation of Bacteriophage DNA by PEG Method   10-DNA isolation by Chelex Method   11-DNA isolation by Lysozyme and Proteinase K   12-Salting-out method for blood samples   13-CTAB or SDS based isolation of plant’s DNA   14-DNA extraction by spooling method   15-Magnetic bead-based nucleic acid isolation   16-Density gradient-based nucleic acid isolation   17-DNA extraction from agarose gel though paper-strips   18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS   19-GENE CLONING AND VECTORS   20-POLYMERASE CHAIN REACTION (PCR)   21-Southern and Northern Blotting   22-GENOME MAPPING   23-Applications of DNA Sequencing Technologies for Current Research

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Author Information

Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).

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