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OverviewFull Product DetailsAuthor: Andrew Currin , Neil SwainstonPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2022 Volume: 2461 Weight: 0.559kg ISBN: 9781071621547ISBN 10: 1071621548 Pages: 278 Publication Date: 23 June 2023 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsDesigning Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach.- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR).- Non-Degenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization.- Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries.- SpeedyGenesXL An Automated, High-Throughput Platform for the Preparation of Bespoke Ultra-Large Variant Libraries for Directed Evolution.- Facile Assembly of Combinatorial Mutagenesis Libraries using Nicking Mutagenesis.- GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes.- Rapid Cloning of Random Mutagenesis Libraries using PTO-QuickStep.- Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs.- Application of Restriction Free (RF) Cloning in Circular Permutation.- Site-Directed Mutagenesis Method Mediated by Cas9.- Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity.- A Screening Method for P450 BM3 Mutant Libraries using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds.- Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method.- Learning Strategies in Protein Directed Evolution.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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