Overview

A team of experts comprehensively review the theoretical and practical aspects of applying antibodies in both the laboratory and clinic. For general understanding, the book thoroughly introduces the basic science of the antibody molecule, including recombinant engineering. Several novel in vivo applications of therapeutic antibodies are then presented to illustrate their special value in varied clinical settings. For those new to the field, there are readily reproducible methods for generating and purifying antibodies and for modifying them for clinical application. Timely and comprehensive, Diagnostic and Therapeutic Antibodies offers today's researchers a concise introduction to the field, as well as a highly useful compendium of practical protocols that will greatly facilitate the application of these powerful new diagnostic and therapeutic agents.

Full Product Details

Author:   Andrew J.T. George ,  Catherine E. Urch
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   40
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.863kg
ISBN:  

9780896037984

ISBN 10:   0896037983
Pages:   477
Publication Date:   01 August 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The Antibody Molecule.- Polyclonal and Monoclonal Antibodies.- Engineering Antibody Molecules.- Phage Display Technology.- Antibodies in Medicine.- Prospects for the Application of Antibodies in Medicine.- Antibodies for Neoplastic Disease.- The Application of Monoclonal Antibodies in the Treatment of Lymphoma.- Antibodies for Inflammatory Disease.- Antibodies for Inflammatory Disease.- Antibodies for Transplantation.- Antibody-Based Therapies in Infectious Diseases.- Antibodies in Nuclear Medicine.- Animal Models for Tumor Localization.- Antibodies for Immunoassays.- Ethics and Industry.- Intellectual Property.- From Laboratory to Clinic.- Production and Purification.- Production of Monoclonal Antibodies.- Purification of Monoclonal Antibodies Using Protein A/G.- Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography.- Quality Control of Raw Materials.- Cell Banks and Stability of Antibody Production.- Measurement of Endotoxin.- Aseptic Vial Filling.- Measurement of Antibody Concentrations by Hemagglutination.- Modification of Antibodies.- Enzymatic Digestion of Monoclonal Antibodies.- How to Make Bispecific Antibodies.- Radiolabeling of Monoclonal Antibodies.- Determination of the Immunoreactivity of Radiolabeled Monoclonal Antibodies.- Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions.- Application of Antibodies in Vitro.- How to Set Up an ELISA.- Measurement of HAMA and Anti-Idiotypic Antibodies.- SDS-PAGE and Western Blotting.- Flow Cytometric Analysis.- Immunocytochemistry.- Immunolabeling for Electron Microscopy.- Antibody Engineering.- PCR of the V Region.- Phage Display Technology.

Reviews

...a welcome addition to the clinician's medical library...written by experts in the field and cover a broad selection of issues, ranging from the basic structure of antibodies to intellectual property rights...Although the chapters can stand independently, the editors have made a major effort to cross-index them by giving references to related material in other chapters...this is a comprehensive book that should be useful to anyone interested in the use of antibodies for therapy, diagnosis, or laboratory investigation. As someone who is currently involved in the development of an antibody-based therapy, I found this book to be quite complete and well written. In many ways it can serve as a manual for the development and use of antibody reagents. The chapters are liberally sprinkled with welcome advice... For clinicians, the book can provide a handy reference for the many types of antibody reagents available for clinical use, as well as information on serological assays. This book fills an important niche in the literature on antibody-based therapies, at a time when reagents are finding their way into clinical use in ever-increasing numbers. - Clinical Infectious Diseases

a welcome addition to the clinician's medical library. written by experts in the field and cover a broad selection of issues, ranging from the basic structure of antibodies to intellectual property rights. Although the chapters can stand independently, the editors have made a major effort to cross-index them by giving references to related material in other chapters. this is a comprehensive book that should be useful to anyone interested in the use of antibodies for therapy, diagnosis, or laboratory investigation. As someone who is currently involved in the development of an antibody-based therapy, I found this book to be quite complete and well written. In many ways it can serve as a manual for the development and use of antibody reagents. The chapters are liberally sprinkled with welcome advice. For clinicians, the book can provide a handy reference for the many types of antibody reagents available for clinical use, as well as information on serological assays. This book fills an important niche in the literature on antibody-based therapies, at a time when reagents are finding their way into clinical use in ever-increasing numbers. - Clinical Infectious Diseases

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