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Full Product Details

Author:   Colin R. MacKenzie ,  Birgit Henrich
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2012
Volume:   903
Weight:   1.167kg
ISBN:  

9781493958856

ISBN 10:   1493958852
Pages:   439
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections.- Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots.- Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples.- Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis.- Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens.- The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease.- Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients.- Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance Mediating Mutations in Region V of the 23S rRNA Gene.- Protocols for Detection and Typing of Treponema pallidum Using PCR Methods.- Protocol for the Extraction of DNA from Dried Blood in the Diagnosis of Convenital CMV Infection.- Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis.- Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis.- Protocol for the Detection and Genotyping of Human Papilloma viruses Using a Liquid Bead Microarray Assay (LBMA).- Protocol for the Use of Enzyme-Linked Hybridisation Assays for Genital Ulcer Disease.- Protocol for the Rapid Detection of the Urogential Tract Mollicutes and Chlamydia with Concomitant LGV –(sub)typing.- Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV.- Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer.- Protocol for the Use of Light Upon Extension (LUX) Real-Time PCR for the Determination of Viral Load in HBV Infection.- Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations.- Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens.- Guidelines for the Use of Molecular Biological Methods to Detect Sexually Transmitted Pathogens in Cases of Suspected Sexual Abuse in Children.- Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae.- Protocol for a Facile Multiplex PCR for Multi-Antimicrobial Resistance and Gonococcus Detection.- Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae.- Protocol for the Detection of Human Leukocyte Antigen Variants and Sexually Transmitted Infections: From Genotyping to Immunoassays.- Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-Parameter Flow Cytometry.- Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity.- Self Collection of Specimens for Nucleic Acid-Based Diagnosis of Pharyngeal, Cevicovaginal, Urethral and Rectal Neisseria gonorrhoeae and Chlamydia trachomatisInfections.- Sexually Transmitted Diseases – Reflections on Metaphors and Ethics.

Reviews

From the book reviews: This volume of Methods in Molecular Biology is the first to provide a comprehensive overview of molecular methods for sexually transmitted infections (STI). ... This is a unique addition to the field of STI diagnostics and will be a useful reference for laboratory scientists wishing to incorporate the methods into their laboratories as well as for researchers and clinicians wishing to enhance their understanding of this area. (Ameeta E. Singh, Doody's Book Reviews, February, 2013)

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