Overview

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab.  Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Editionserves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures.

Full Product Details

Author:   Jason M. Hansen ,  Louise M. Winn
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2019
Volume:   1965
Weight:   1.056kg
ISBN:  

9781493991815

ISBN 10:   1493991817
Pages:   436
Publication Date:   09 May 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. In Vitro Models in Developmental Toxicology Louise M. Winn 2. Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments Jason M. Hansen and Ted B. Piorczynski 3. Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology Charles A. Easley 4. Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model Mika Suga and Miho K. Furue 5. Micromass Methods for the Evaluation of Developmental Toxicants Omar J. Mohammed and Margaret K. Pratten 6. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System France-Helene Paradis, Han Yan, Chunwei Huang, and Barbara F. Hales 7. Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology Barbara D. Abbott 8. Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, and Sakhila K. Banu 9. Hepatotoxicity in Zebrafish Larvae Kathryn Bambino, Joshua Morrison, and Jaime Chu 10. Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model Joslynn G. Affleck and Virginia K. Walker 11. The Chicken Embryo as a Model in Developmental Toxicology Michael R. Stark and Micah M. Ross 12. The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity Cecilia Berg 13. Mouse Whole Embryo Culture Emily W.Y. Tung and Louise M. Winn 14. Rat Whole Embryo Culture Craig Harris 15. Rabbit Whole Embryo Culture Terence R.S. Ozolins 16. Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology Brendon K. Warner, Jonathan K. Alder, and Arminda Suli 17. Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish Jonathon T. Hill 18. Assessment of Histiotrophic Nutrition Using Fluorescent Probes Craig Harris 19. Examining Gene Expression Patterns through Whole Mount In Situ Hybridization Jeffery R. Barrow 20. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos Robert M. Zucker and John M. Rogers 21. Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection Danielle M. Drake, Aaron M. Shapiro, and Peter G. Wells 22. Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA Shama Bhatia and Peter G. Wells 23. Western Analysis of Breast Cancer 1 Protein (BRCA1) Danielle M. Drake and Peter G. Wells 24. Regulation and Control of AP-1 Binding Activity in Embryotoxicity Terence R.S. Ozolins 25. Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) Shama Bhatia, Jason Matthews, and Peter G. Wells 26. The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity Rebecca Maciver and Terence R.S. Ozolins 27. Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, and Michelle Graf

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