Overview

The first collection of methods to study embryonic and fetal hematopoiesis in both invertebrate (fruit flies) and vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Full Product Details

Author:   Margaret H. Baron
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2005 ed.
Volume:   105
Dimensions:   Width: 15.50cm , Height: 3.10cm , Length: 23.50cm
Weight:   0.982kg
ISBN:  

9781588292964

ISBN 10:   1588292967
Pages:   480
Publication Date:   15 October 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Genetic Approaches.- Gene Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse.- Inducible Transgene Expression in Mouse Stem Cells.- Quantitative Trait Analysis in the Investigation of Function and Aging of Hematopoietic Stem Cells.- Analysis of Cell Fate Outcomes in Transplantation Models.- Flow Cytometric Analysis of Hematopoietic Development.- Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells.- Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice.- Animal Models.- Hemocyte Development During Drosophila Embryogenesis.- Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos.- Fate Mapping Hematopoietic Lineages in the Xenopus Embryo.- Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain).- Use of Flow Cytometry and Combined DNA Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo.- Analysis of Hematopoietic Development in the Zebrafish.- Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos.- In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo.- Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development.- Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo.- Hematopoietic Stem Cell Development During Mouse Embryogenesis.- Analysis of Hematopoietic Progenitors in the Mouse Embryo.- In Vivo and In Vitro Assays of Thymic Organogenesis.- Retroviral Transduction in Fetal Thymic Organ Culture.- Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors.- ANew Clonal Assay System for Lymphoid and Myeloid Lineages.- Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture.- Analysis of the Vascular Potential of Hematopoietic Stem Cells.- Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture.- Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation.- Analysis of Hematopoietic Development During Human Embryonic Ontogenesis.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- Bioinformatics and Functional Genomics.- A Functional Genomics Approach to Hematopoietic Stem Cell Regulation.

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