Overview
This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Full Product Details
Author: Julie Maupin-Furlow , Mariola J. Edelmann
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 1st ed. 2023
Volume: 2591
Weight: 0.794kg
ISBN: 9781071628027
ISBN 10: 107162802
Pages: 300
Publication Date: 10 November 2022
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
1. Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach Zhengrui Zhang and Chittaranjan Das 2. Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs Max S. Kloet and Gerbrand J. van der Heden van Noort 3. Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell Alireza Rahnama and Adam T. Melvin 4. Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue Bindu Nanduri, Leslie A Shack, John Santelices, and Mariola J Edelmann 5. Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases Wioletta Rut, Mikolaj Zmudzinski, and Marcin Drag 6. High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity Gang Xie, Ken C Dong, Evan J Worden, and Andreas Martin 7. Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels Hannah B. L. Jones, Raphael Heilig, Benedikt M. Kessler, and Adán Pinto-Fernández 8. High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery Virginia De Cesare and Paul Davies 9. Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases Thomas Hermanns and Kay Hofmann 10. Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay Nathaniel Hepowit and Julie A. Maupin-Furlow 11. Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease Digant Nayak, Priscila dos Santos Bury, Marcin Drag, Alfred N. Nijkerk, Farid El Oualid, and Shaun K. Olsen 12. Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases Anastasios Spiliotopoulos, Sigrun K. Maurer, Maria T. Tsoumpeli, Juan A. F. Bonfante, Jonathan P. Owen, Kevin C. Gough, and Ingrid Dreveny 13. Identification of Deubiquitinase Substrates in Xenopus Egg Extract Valentina Rossio, Joao A. Paulo, and Randall W. King 14. Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression Heather E. Arsenault and Jennifer A. Benanti 15. Real-time Deubiquitination Assays using a Free-Ubiquitin Sensor Yun-Seok Choi and Robert E. Cohen 16. A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells Christopher Rainville, David E. Sterner, and Kumar Suresh 17. Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast Nathaniel L. Hepowit, Aeva S. Denise, and Jason A. MacGurn
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